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Blood Collection Fill In The Blanks

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Question: COLLECTION INVOLVES: Answer: • Serum/plasma/whole blood • Animal • Syringe • Vacutainer
Question: WHAT IS AN ? Answer: • Work by calcium (factor 4) • Chemical when added to blood will prevent or delay coagulation • Can come as liquid or powder form in tubes
Question: RED TOP BLOOD COLLECTION Answer: NO
Question: LAVENDER TOP BLOOD TUBEAnswer: Contains EDTA o Use for CBC o Good for staining o Cells will shrink (crenation) in here if too blood (due to salt in EDTA)
Question: GREEN TOP COLLECTION TUBEAnswer: (sodium or lithium) o One of the most common tubes o Not routine in CBC = clumps WBC o Not used for staining o Birds, reptiles, fish = RBC have nucleolus so the cell will not lyse
Question: LIGHT BLUE TOP BLOOD TUBEAnswer: buffered sodium citrate (just delays the clotting) o Used for study
Question: GRAY TOP COLLECTION TUBE Answer: contains anticoagulant and glycolytic inhibitor (prevents of glucose) o Sodium fluoride
Question: YELLOW TOP COLLECTION TUBEAnswer: ACD: Acid Citrate Dextrose o ACD used all the time when blood transfusions
Question: BLOOD COLLECTION IS DETERMINED BY:Answer: o Test o Tube size o Animal size & hydration – will effect the volume of blood you can attain
Question: MAXIMUM BLOOD SHOULD NOT EXCEED:Answer: 25% of Body o Birds – only 1% of BW
Question: PCV IS: Answer: PACK CELL : 50% of your blood is cells (RBC)
Question: BLOOD COLLECTION SITESAnswer: vein o Facial vein
Question: BLOOD COLLECTION SITES Answer: o Jugular vein o Coccygeal vein o Mammary – has a higher natural level of calcium – LAST
Question: OVINE BLOOD SITESAnswer: o Jugular o Saphenous
Question: CAPRINE BLOOD COLLECTION Answer: o Jugular o Saphenous
Question: PORCINE BLOOD SITES Answer: o Thoracic Inlet – anterior vena cava
Question: CANINE COLLECTION SITES Answer: o Cephalic o Lateral Saphenous
Question: FELINE BLOOD COLLECTION Answer: o Jugular o Cephalic Saphenous
Question: BIRD BLOOD SITES Answer: right jugular vein (left is smaller), medial metatarsal vein, wing vein (cutaneous vein)
Question: FERRET COLLECTION SITES Answer: cranial vena cava, ventral tail
Question: RABBIT COLLECTION SITES Answer: lateral saphenous, lateral ear vein (not preferred due to hematoma forming), cephalic vein, not jugular because of
Question: SMALL ANIMAL BLOOD SITES Answer: JUGULAR
Question: RODENT BLOOD COLLECTION Answer: saphenous, venous sinus (behind eye), tail vein, submandibular, direct cardiac puncture
Question: EXOTICS BLOOD COLLECTION Answer: o Lizards: ventral to tail vein /Turtle: venous sinus o Snakes: direct cardiac puncture
Question: NEEDLES Answer: • Length ½” to 2” • Size range: 14g to 30g (smaller #, the needle) • Don’t use smaller than 25g needle – this will cause hemolysis (lyse blood cells)
Question: SIZES Answer: o 18g – green monoject – pink B-D o 20g – pink monoject – yellow B-D o 21g – lavender monoject – green B-D o 22g – blue – black B-D o 23g – orange monoject – teal B-D o 25g – red monoject – blue B-D
Question: SYRINGE Answer: o 1cc, 3cc, 6cc, 12cc, 35cc,
Question: TIPS Answer: (needle slips on), luer lock (needle screws on), catheter (shaped like a cone), curved (can feed animals with this, flush wounds) o Location of the tip can be: Central vs. eccentric
Question: SYSTEM Answer: parts: o Double ended needle o Plastic sleeve o Vacuum tube
Question: DRAW TECHNIQUE Answer: • Choose tube, needle, syringe • Label tube(s) • Proper restraint • Locate vein • Insert needle bevel up • Draw back on plunger • Place blood in tube gently – remove top of tube and and drip the blood into the tube – this prevents lysis
 
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Created by: jenn.messier11
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