In each blank, try to type in the
word that is missing. If you've
typed in the correct word, the
blank will turn green.
If your not sure what answer should be entered, press the space bar and the next missing letter will be displayed. When you are all done, you should look back over all your answers and review the ones in red. These ones in red are the ones which you needed help on. Question: COLLECTION INVOLVES: Answer: • Serum/plasma/whole blood
• Animal
• Syringe
• Vacutainer Question: WHAT IS AN ? Answer: • Work by calcium (factor 4)
• Chemical when added to blood will prevent or delay coagulation
• Can come as liquid or powder form in tubes Question: RED TOP BLOOD COLLECTION Answer: NO Question: LAVENDER TOP BLOOD TUBEAnswer: Contains EDTA
o Use for CBC
o Good for staining
o Cells will shrink (crenation) in here if too blood (due to salt in EDTA) Question: GREEN TOP COLLECTION TUBEAnswer: (sodium or lithium)
o One of the most common tubes
o Not routine in CBC = clumps WBC
o Not used for staining
o Birds, reptiles, fish = RBC have nucleolus so the cell will not lyse Question: LIGHT BLUE TOP BLOOD TUBEAnswer: buffered sodium citrate (just delays the clotting)
o Used for study Question: GRAY TOP COLLECTION TUBE Answer: contains anticoagulant and glycolytic inhibitor (prevents of glucose)
o Sodium fluoride Question: YELLOW TOP COLLECTION TUBEAnswer: ACD: Acid Citrate Dextrose
o ACD used all the time when blood transfusions Question: BLOOD COLLECTION IS DETERMINED BY:Answer: o Test
o Tube size
o Animal size & hydration – will effect the volume of blood you can attain Question: MAXIMUM BLOOD SHOULD NOT EXCEED:Answer: 25% of Body
o Birds – only 1% of BW Question: PCV IS: Answer: PACK CELL : 50% of your blood is cells (RBC) Question: BLOOD COLLECTION SITESAnswer: vein
o Facial vein Question: BLOOD COLLECTION SITES Answer: o Jugular vein
o Coccygeal vein
o Mammary – has a higher natural level of calcium – LAST Question: OVINE BLOOD SITESAnswer: o Jugular
o Saphenous Question: CAPRINE BLOOD COLLECTION Answer: o Jugular
o Saphenous Question: PORCINE BLOOD SITES Answer:
o Thoracic Inlet – anterior vena cava Question: CANINE COLLECTION SITES Answer:
o Cephalic
o Lateral Saphenous Question: FELINE BLOOD COLLECTION Answer: o Jugular
o Cephalic Saphenous Question: BIRD BLOOD SITES Answer: right jugular vein (left is smaller), medial metatarsal vein, wing vein (cutaneous vein) Question: FERRET COLLECTION SITES Answer: cranial vena cava, ventral tail Question: RABBIT COLLECTION SITES Answer: lateral saphenous, lateral ear vein (not preferred due to hematoma forming), cephalic vein, not jugular because of Question: SMALL ANIMAL BLOOD SITES Answer: JUGULAR Question: RODENT BLOOD COLLECTION Answer: saphenous, venous sinus (behind eye), tail vein, submandibular, direct cardiac puncture Question: EXOTICS BLOOD COLLECTION Answer: o Lizards: ventral to tail vein /Turtle: venous sinus
o Snakes: direct cardiac puncture Question: NEEDLES Answer: • Length ½” to 2”
• Size range: 14g to 30g (smaller #, the needle)
• Don’t use smaller than 25g needle – this will cause hemolysis (lyse blood cells) Question: SIZES Answer: o 18g – green monoject – pink B-D
o 20g – pink monoject – yellow B-D
o 21g – lavender monoject – green B-D
o 22g – blue – black B-D
o 23g – orange monoject – teal B-D
o 25g – red monoject – blue B-D Question: SYRINGE Answer: o 1cc, 3cc, 6cc, 12cc, 35cc, Question: TIPS Answer: (needle slips on), luer lock (needle screws on), catheter (shaped like a cone), curved (can feed animals with this, flush wounds)
o Location of the tip can be: Central vs. eccentric Question: SYSTEM Answer: parts:
o Double ended needle
o Plastic sleeve
o Vacuum tube Question: DRAW TECHNIQUE Answer: • Choose tube, needle, syringe
• Label tube(s)
• Proper restraint
• Locate vein
• Insert needle bevel up
• Draw back on plunger
• Place blood in tube gently – remove top of tube and and drip the blood into the tube – this prevents lysis |
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