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Stack #160523
Microbiology Test #2 Review Dr. Bharathi
Question | Answer |
---|---|
What is an organism that produces superoxide dimutase and catalase? | Aerobe |
What is not a disadvantage for a direct microscope count? | Sample volume of an unknown. |
What are the disadvantages of direct microscope count? | Motile bacteria are difficult to count, dead cells are likly to be counted, high concentrations of cells are requred to be countable. |
What is the advantage of direct microscope count? | No incubation time is required. |
What do hydrothermal vents on the ocean floor useas a carbon source? | CO2 |
What is in nutrient agar? | peptone & beef extract |
What do producers in the vents use? | sulfides |
What is the direct method to count microbial growth? | metabolic activity |
What is the best pH for most bacteria? | 7 |
What is the best temperature for optimum growth of mesophiles? | 25 |
What is the best temperature for optimum growth of psychrophiles? | 15 |
What is the best temperature for optimum growth of psychotrophs? | 20-30 |
What catalyzes 2H202---into 2H2O + O2? | Catalase |
What is an organic growth factor? | NAD+ |
What is not used to determine the metabolic growth factor? | Turbidity |
What is an organism that uses oxygen or grows without oxygen? | facultative anaerobe |
What is not a disadvantage to determine viable cells? | viable plate count |
How do salts and sugars preserve food? | creates a hypertonic environment |
What makes H202 + 2H+ ----into 2H20? | peroxidase enzyme |
What is a common intermediate between catabolic and anabolic pathways? | ATP |
When are gram positive bacteria most susceptible to penicillin? | Log phase |
What organisms are capable of respiration? | anaerobic |
What is generated by the flow of protons across the cell membrane? | ATP |
What neutralizes acids in the medium? | buffers |
What measures microbial growth? | metaboic activity & glocose consumption |
Inwhat mediums do gram positive organisms grow such as staphylococcus aureus colonies? | selective & differential |
How do gram positive organisms grown in selective & differential mediums look? | yellow halo surrounds |
What is a step in glycolysis? | oxidation |
What do aeobic and anaerobic respiration have in common? | final receptor of electrons are different in both |
What is the oxidation of glucose with organic molecules serving as electron receptors? | fermintation |
What is not necessary for respiration? | oxygen |
What is an example of cyanobacteria? | photoautotroph |
What uses CO2 for carbon and H2 for energy? | chemoautotroph |
What is involved in aerobic respiration and oxidized in the Krebs Cycle? | pyruvic acid |
What happens to a bacterial culture grown in glucose? | peptide medium causes the pH to increase most likely using peptides |
What does phosphoeno pyruvatie into pyruvic acid generate? | ATP |
What is happening when ATP general when protons are moved across a membrane? | chemiosmotic mechanism |
What does photophosphrylation require? | CO2 |
What is not an enzyme? | Coenzyme A |
What is not an end product of fermentation? | Pyruvic acid |
What has bacteria chlorophylls and uses alcohols for carbon? | photoheterotrophs |
Fermentation is defined as what? | Metabolic process |
Fermentatin releases what? | energy from sugar |
Fermentation does not require what? | oxygen |
How does fermentation occur? | anaerobically |
What is the oxidation of intrmediate metabolic compounds? | substrate level phosphorylation |
What is metabolized in the Krebs Cycle or citric acid cycle? | fattyacids |
What is the energy released as carrier molecules and are oxidized and used to generte ATP? | oxidative phoshorylation |
What does not produce 3 ATPs? | pentose hophate pathway |
What uses glycose for carbon and energy? | chemoheterotroph |
How does a strictly fermentative bacteria produce energy? | glycolyis only |
What is not an energy source for a photautotroph? | CO2 |