What is the purpose of flaming the mouth of the tube?

Convection. To prevent airborne contamination.

What other methods can be used to determine motility?

A flagella stain. A microscope. Motility agar.

What is the primary use of slants?

Long-term storage. Pure cultures.

What is the primary use of deeps?

To store anaerobic bacteria. To test oxygen requirements.

What is the primary use of broths?

To grow large amounts of bacteria.

What is the purpose of flaming the loop before and after use?

To sanitize the loop. To incinerate anything that is on the loop.

Why must the loop be cool before you touch it to a culture?

To avoid killing the organisms.

How do you determine when the loop is cool?

You will hear a sizzle if it is not. Trial and error. Practice.

When is a loop preferable for transferring bacteria?

When you are transferring bacteria to a plate, a slant, or broth.

When is a needle preferable?

When you are transferring bacteria to a deep.

Why is aseptic technique important?

To prevent contamination and disease.

For a bacterium, what evolutionary advantage is associated with forming a pellicle in a liquid medium?

If the bacteria is aerobic, it floats to the top.

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Micro Lab Exercise 4

Question	Answer
Bacteria that are introduced into various forms of culture media to keep them alive and to study their growth without introducing unwanted microbes.	Inoculated
Unwanted microbes	Contaminants
Technique used in microbiology to exclude contaminants.	Aseptic Technique
Rendered free of all life	Sterilized
Test tubes containing solid culture media that were left at an angle while the agar solidified. Easier to store and transport.	Agar Slants
Agar is allowed to solidify in the bottom of a test tube. Often used to grow bacteria that require less oxygen than is present on the surface of the medium.	Agar deep
A sterile, heat-resistant, noncorroding Nichrome wire attached to an insulated handle. When the end is bent into a loop it's called?	Inoculating loop
A sterile, heat-resistant, noncorroding Nichrome wire attached to an insulated handle. When it's straight it's called?	Inoculating needle
For special purposes, sterile swabs, pipettes, glass rods, or syringes may be used to transfer cultures.	True
What is the purpose of flaming the mouth of the tube?	Convection. To prevent airborne contamination.
What other methods can be used to determine motility?	A flagella stain. A microscope. Motility agar.
What is the primary use of slants?	Long-term storage. Pure cultures.
What is the primary use of deeps?	To store anaerobic bacteria. To test oxygen requirements.
What is the primary use of broths?	To grow large amounts of bacteria.
What is the purpose of flaming the loop before and after use?	To sanitize the loop. To incinerate anything that is on the loop.
Why must the loop be cool before you touch it to a culture?	To avoid killing the organisms.
Should you set down the loop to let it cool?	No. It will get contaminated.
How do you determine when the loop is cool?	You will hear a sizzle if it is not. Trial and error. Practice.
When is a loop preferable for transferring bacteria?	When you are transferring bacteria to a plate, a slant, or broth.
When is a needle preferable?	When you are transferring bacteria to a deep.
Why is aseptic technique important?	To prevent contamination and disease.
For a bacterium, what evolutionary advantage is associated with forming a pellicle in a liquid medium?	If the bacteria is aerobic, it floats to the top.
How can you tell that the media provided for these exercises were sterile?	No turbidity, clear.

Created by: KamiSeago

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