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micro lab# 10 11

lab definitions

QuestionAnswer
inoculation intentional introduction of microbes onto nutrient agar or nutrient broth
inoculating loop used to transfer bacteria from a solid medium to broth or from broth to broth
inoculating needle is used to inoculate deep or pick up small isolated colonies from a solid
incubation period time needed for bacteria to grow
turbid/turbidity cloudiness that appears in clear nutrient broth to indicate the growth of bacteria presence of turbidity indicates the growth of bacteria
pellicle "skin" that forms on the top of the broth used by aerobes to stay close to the surface to obtain oxygen they need
motility ability to move demonstrated by an "inverted christmas tree" shape in the deep indication that bacteria moved away from the stab line into the agar
streak horizontal movements in a back & forth fashion made on the petri plate to inoculate agar & spread out bacteria to allow for growth into colonies
broth culture liquid medium used to grow large numbers of bacteria
agar slant solid medium used to store pure cultures
agar deep solid medium used to store anaerobic bacteria and or test for oxygen requirements
colony population of cells that arises from one bacterial cell
aseptic technique used in microbiology to exclude contaminants
sterilized rendered free of all life usually accomplished by autoclave
streak plate a mixed sample of bacteria is streaked over the surface of a solid medium many times
pure culture one containing a single kind of microbe
Created by: Caya Belton
 

 



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