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Histotechnology

Lecture 1

QuestionAnswer
What is the meaning of the term histology? Greek: "histos" - tissue; "logos" - study of.
H&E is a routine stain in histology. What does the acronym mean? Haematoxylin & esoin.
What does the haematoxylin stain and why? Haematoxylin is a basic dye that stains acidic structures purplish-blue. Nuclei, ribosomes and rER have an affinity for haematoxylin because of their high DNA and RNA content.
What structures does esoin stain and why? Eosin is an acidic dye that stains basic structures red or pink. Most cytoplasmic proteins are basic, thus the cytoplasm usually stains pink.
In histology, if the information on the specimen and the requisition do not match we discard that sample. Correct? No, Histology is an exception. Many samples may not be retrievable. Follow your laboratory's SOP to determine the correct course of action.
The pathology laboratory recieves specimens from where? A specimen can include almost anything removed from the body. Specimens may come from the operating room, day surgery, clinics or doctors offices.
What is the difference in fixation time between gross and biopsy specimens? Gross (large) specimens must be fixed overnight due to their size. Biopsies can be fixed & processed right away because they are smaller.
Name five different methods of obtaining a biopsy (Bx) specimen. 1. forceps at the end of an endoscope; 2. shave Bx - slices of surface tissue (skin); 3. punch Bx - piercing tissue with a needle (skin or cervix); 4. cone biopsy - cone shaped tissue removed (cervix); 5. core Bx -needle through skin into organ/tissue
An adequate gross (macroscopic) description includes which 5 pieces of information? 1. number of pieces; 2. measurement in three dimensions (size/weight/volume); 3. appearance/colour; 4. consistency (soft, hard, rubbery, fleshy, etc.); 5. other abnormalities (lesions, tumors, etc.) described in detail.
Created by: MLT_NSCC
 

 



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