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Micro Lab Part 1
Lab 1 Microscopy
| Question | Answer |
|---|---|
| This objective lens provides the highest magnification | Oil immersion |
| This objective lens provides the second highest magnification | High-dry |
| This objective lens provides the lowest magnification | Low Power |
| This objectivie lens has the shortest working distance | oil immersion |
| The coarse focus knob should be adjusted only when using this objective lens | low power |
| This lens collects and focuses light from the lamp onto the specimen on the slide | condenser |
| This lens, als known as the eyepiece, often comes in pairs | ocular |
| Diopter adjustments can be made to this lens | ocular |
| A diaphragm is used to regulate light passing through this lens | condenser |
| Acetone is the safest solvent for cleaning an objective lens, T or F? | False |
| Only lint-free, optically safe tissue should be used to wipe off microscope lenses | True |
| The total magnification capability of a light microscope is only limited by the magnifying power of the lens system | FALSE |
| The coarse focus knob can be used to adjust the focus when using any of the objective lenses. | False |
| Once focus is achieved at one magnification, a higher-power objective lens can be rotated into position without fear of striking the slide. | True |
| The resolving power of a microscope is a function of;a)the magnifying power of the lensesb)the numerical aperture of the lensesc)the wavelength of light. d)Both (a) and (b) are correct.e)Both (a) and (c) are correct. | E |
| The coarse and fine focus knobs adjust the distance between;a)the objective and ocular lensesb)the ocular lensc)the ocular lenses and your eyesd) the stage and the condenser lense) the stage and the objective lens | E |
| A microscope that maintains focus when the objective magnifcation is increased is called; | parafocal |
| The total magnification achieved when using a 100x oil immersion lens with 10X binocular eyepeice is; | 1000X |
| The most useful adjustment for increasing image contrast in low-power magnification is; | Closing down the diaphram |
| Before the oil immersion lens is rated into place you should; | Center the object of interest in the preceding lens and place a drop of oil on the slide |
| At 10X, 100X total magnification, what is the size of one unit? | 10 um |
| At 40X, 400X total magnification, what is the size of one unit? | 2.5 um |
| At 100X, 1000X total magnification, what is the size of one unit? | 1 um |
| The ability to make larger is | magnification |
| The ability of the lenses to distinguish two points is; | resolution |
| What type of microscope is used to view bacteria? | A compound microscope |
| Total magnification = | Objective lens x ocular lens |
| The light bending ability of a medium is the | refractive index |
| What is used to keep light from bending? | Immersion oil |
| What are the two steps of Specimen preparation? | 1. Thin film of a solution of microbes on a slide is a smear.2.) A smear is usually fixed to attach the microbes to the slide and to kill the microbes. |
| In a basic dye, the chromophore is a | cation |
| In an acidic dye, the chromophore is a | anion |
| Staining the backround instead of the cell is called; | negative staining |
| Simple stain | Use of a single basic dye such as methyleneblue, carbolfuscin, crystal violet |
| Differential Stains | Use two or more stains and categorize cells into groups; Gram Stain and Acid-Fast Stain |
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