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6 major groups of microorganisms
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MICROBIOLOGY QUIZ 1

QuestionAnswer
6 major groups of microorganisms Algae, Bacteria, Fungi, Helminths, Protozoa, and Viruses
1 micrometer= 10 to the negetive 9th meter
virus's are in what meter range? nanometer
how many meters are virus's 10 to the negative 9th
prokaryotes have no nucleus
3 eukaryotes fungus protozoa and algea
central dogma dna is turned to rna which then goes to proteins
archaea are made up of eukaryotes
why is archaea different from bacteria because they live in very hot climates
3 domains bacteria archaea and eukaryotes
how old are microorganisms 4 million years old
when is first fossil evidence 3.5 billion years ago
first microorganisms are responsible for creating aerobic enviroment (oxygen containing enviroment)
what is first bacteria and what did it do cyanobacteria, 3.5 billion years ago, helped create oxygen
cyano bacteria are the largest.. portion of biomass on earth
cyanobacteria allowed other organisms to start flourishing
what is base of foodchain microorganisms
alexander fleming discovered pennesilin
what about pennesilin? penicillium colony stopped growth of bacteria- created a halo around colony
microorganisms can break down pollutants in enviroment- bioremediation
a lot of organisms able to convert __ into __ from ___ corn into ethanol from fermentation
food is spoiled by microorganisms
what helps preserve food? heat, cold, radiation, and chemicals
monosodium, glutamate, critric years are all microorganisms that work with food
microorganisms can be disease agents through biotechnology
insulin is produced from __ and is put into microorganisms to liver of pigs, manipulate cells
microorganisms can be used in gene __ therapy
gene therapy example cystic fibrosis- cant breathe properly because of mucus- caused by single mutation in gene so inject them with protein which allows them to overcome disease
biotechnology is manipulating organisms
hooke first person to discover idea of cells
who looked at a cork and developed the word "Cells" hooke
what year did hooke progress in? 1665
what was anton von leewekhoek interested in? looking at cloth under a microscope.. then salivia and pond water
what did leewekhoek discover? "animalcules" which are known as bacteria now
what year did leewekhoek make discoveries? 1673
pasterurs experiments involved disproving spontaneous generation theory in 1861
how did pasterurs disprove spontaneous generation theory? took broth and boiled it so everything was killed but eventually if left open the broth will become contaminated and microbacteria will pepopulate
thus, pasterurs proved that living matter does not come from nonliving matter
pasterurs also famous for achieving vaccines for anthrax and rabies
who discovered that bacteria causes wine spoilage? pasterurs
who created sterilization and pasteurization? pasterurs
dead zones heat causes algae which are on top of pollutants like fertilizers are food for it, algea bloom , when they die get decomposed by microorganisms, uses up all oxygen
what causes a deadzone? combination of pollutants and run off and algea feeding off of it
first step in stopping deadzones? controlling the pollutants going into the water
bassi realized that silkworm disease was caused by certain type of fungus
berkely found that the great potato blight was caused by fungus
lister realized from pesters work that with use of __ he could antiseptic agents he steralized his intruments and mortality rate went up
koch was first scientist to demonstrate directly the role of bacteria in causing the diseas anthrax
anthrax is caused by bacillus anthracisi
how did koch demonstrate anthax findings? culture microorganisms from dead cows and inject it to healthy ones and healthy animals died
what was this called? the germ theory
that did edward jenner do? smallpox vaccine
how we use microorganisms medically medical microbiology
related to microbiology bc looking how microorganisms trigger immune responses ummunology
looking at microorganisms in our enviroment microbial ecology
dna manipulation from one organism to another- the branch of microbiology where we manipulate genos with microorganisms microbial genetics
taking bits of dna from one organism and giving it to another organisms genetic engineering
genetic engineering allows for thing to resist its enviroment
rna into protein is carried by ribosomes- called translation
dna to rna is called transciption
paramecium- protozoan
with brightfield uses light, must stain cells, organisms will be dead after staining them
darkfield use condenser, used to look at organisms that dont stain well
example of organism used under darkfield tuberculosis bc doesnt stain wel, smaller cells, like syphilis
are cells still dead under darkfield? yes
phase contract do not stain the cells so cells are alice
basic stains have a __ charge and consist of (3) positive- crystal violet, methylene blue, safranin
organisms have slight __ charge so negative charge so stain is attracted to cells and will bind with them
differential stains: you can differentiate between one type of one organisms to another- one that is typically used with a gram stain
all bacteria fall into 2 groups- one that stain with color with gram stain and another that gives another color
what does this depend on? structure of cell
gram stain uses different stypes of stain- crystal violet, iodine, acohol, and safranin
with crytal violet all cells look purple
then you apply iodine
iodine helps crytal violet enter into cells
crystal violet is a mordant
alcohol __ crystal violet from cells removes
then you need a __ which is usually counter stain, safranin
gram pos vs gram neg positive are ones who retain crystal violet, negative are those who lost violet and gain color from safranin
use color to differentiate between different cells
all cells either gram positive or gram negative
2 examples of negative staining klebsiella, pnemoniae
why are these negative staining have capsules which have slimy layer around cell prevent stain
capsules cause pnemonia to be pathogenic
endospore staining spores found inside cell, very difficult to stain
example of endorspore staining vacillus anthracis, anthrax
electron microscopes have far greate ___ than light microscopes.. approx __ resolving power; .2 nm
electron microscopes are good for seeing viruses and on and inside the cells
distorations are artifacts- might not be present in cells
how does distorations happen? electron hitting valleys and troughs, if its a tough secondary electrong that got knocked off arnt going far enough to create an image
get light and dark images depending on structure of cell
tem image binary fission- bacterial cell, dna
transmission microscope you can see inside the organisms
transmission electron microscope zoom 10,000 to 100,100 x
scanning electron microscope 3 diminsenial imaging and scanning surface
scanning electron microscope zoom 1000 10,000 x
microorganisms elemt cycling includes conps
Created by: lilcollins92
 

 



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