Question | Answer |
What anticoagulant is used to make peripheral blood smears? | EDTA |
What will stain dark blue (or purple) using a Wright-Giemsa stain? | Cell nucleus |
Which term means an increase in the variation of cell size? | Anisocytosis |
In performing a platelet estimate at 1000x magnification, the technologist sees an average of 20 platelets per field. How would you interpret this? | Normal |
What term means an increase in the average size of erythrocytes? | Macrocytosis |
What term means a decrease in the average size of erythrocytes? | Microcytosis |
In performing a platelet estimate at 1000x magnification, the technologist sees an average of 41 platelets per field. How would you interpret this? | Increased |
When would a tech choose to count 200 WBCs for a differential instead of the normal 100? | If the WBC count is greater than 40.0x10^9/L |
The instrument reports a total WBC of 133.8 x 10^3/uL but unable to read the % of the various WBCs due to the high WBC count. Perform a manual diff and find 13% segs, 74% lymphs, 8% monos, 2% eos, and 3% basophils. What is the absolute neutrophil count? | 17.4 x 10^3/uL |
The instrument reports a total WBC of 133.8 x 10^3/uL but unable to read the % of the various WBCs due to the high WBC count. Perform a manual diff and find 13% segs, 74% lymphs, 8% monos, 2% eos, and 3% bas. What is the absolute monocyte count? | 10.7 x 10^3/uL |
What percentage of red cells are destroyed and replaced daily in a normal adult? | 1% |
The best definition of anemia is: | A decrease in the oxygen carrying capacity of the blood |
What lab results would suggest anisocytosis? | RDW= 18.0 |
What lab results would suggest a decreased red blood cell count? | HCT= 30 |
The presence of bluish red blood cells is termed: | polychromasia |
Renal disease causes a: | normocytic, normochromic anemia |
Iron deficiency causes a: | microcytic, hypochromic anemia |
Sideroblastic anemia causes a: | microcytic, hypochromic anemia |
Manual cell counting methods in automated hematology laboratories are used for all of the following: | very low counts, malfunctioning automated instruments, and counting spinal fluids
does NOT include: routine testing |
In performing a manual RBC count, the tech counts an average of 8 red blood cells. The specimen was undiluted. What is the RBC count for this specimen? | 400 RBCs/uL
8/5 x 0.004= 8/.02 |
In performing a manual WBC count, the tech counts 155 WBCs on one side and 151 WBCs on the other. The specimen was diluted 1:10. What is the WBC count for this specimen? | 3825 WBCs/uL
((155+151)/2) x 10 / 4 x 0.1 |
In performing a manual WBC count, the tech counts an average of 16 WBCs. The specimen was counted undiluted. What is the WBC count for this specimen? | 40 WBCs/uL |
Twenty cells are counted in one of the nine large squares of a hemocytometer. The sample is diluted 1:10. How many cells are present per mL? | 2,000 |
On a standard reticulocyte preparation with new methylene blue, there are 100 cells counted with blue-stained granulofilamentous material. The red blood count is 3.22 x 10^9/uL and the hematocrit is 30%. Calculate the reticulocyte count as a percent. | 100 retics/ 1000 RBCs = 1/10= 10% |
What is a good use of the erythrocyte sedimentation rate (ESR)? | monitor patients with rheumatoid arthritis |
What can cause a falsely increased erythrocyte sedimentation rate (ESR)? | tube not totally vertical
-this applies to traditional ESRs
--westergren
--wintrobe
--modified wintrobe |
How do sickle cells or spherocytes interfere with the erythrocyte sedimentation rate (ESR)? | prevent rouleaux formation so falsely decrease |