Unit 1 lab techniques
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| show | 1ml of stock solution with 9ml of water. Then 1ml of diluted solution with 9ml of water and so on....
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| What substance controls pH | show 🗑
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| Describe two techniques that can be used to quantify the concentration of an unknown solution | show 🗑
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| show | spectrophotometer
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| What is a standard curve | show 🗑
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| On what property are substances separated in centrifugation | show 🗑
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| What name is given to the substance at bottom and top during centrifugation | show 🗑
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| Small substances are found where after centrifugation | show 🗑
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| show | Iso electric focusing
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| What is meant by the iso electric point of a protein | show 🗑
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| What are the two types of gel electrophoresis by which proteins can be separated | show 🗑
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| show | All proteins are denatured to give a negative charge and move to positive end dependent on size
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| show | Proteins remain in original conformation (no denaturing) and migrate through gel dependent on SIZE & CHARGE
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| show | Denaturing (as all proteins are artifically made negative)
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| What type of gel electrophoresis depends on size and charge | show 🗑
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| show | size of pores in buffer
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| What are the types of microscopy | show 🗑
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| show | Fluorescence
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| show | Bright field
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| Which type of microscopy is more simple to use | show 🗑
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| Which type of microscopy involves antibodies to tag objects | show 🗑
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| Name an object that can be viewed under a bright field microscope | show 🗑
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| show | Total cell count
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| What technique is able to distinguish between dead cells and viable cells | show 🗑
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| Explain why only dead cells stain with trypan blue | show 🗑
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| Explain how to estimate total cell count in a haemocytometer | show 🗑
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| What term is given to cells that can only divide a limited number of times (hayflick) limit | show 🗑
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| What term is given to cells that can divide infinitely | show 🗑
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| show | To enable explant to divide into callus AND to enable callus to differentiate into plantlet
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| Why do mammalian cells need foetal bovine serum | show 🗑
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| Why do mammalian cells need antibiotics | show 🗑
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| show | microbes
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| show | plants
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| Describe the 5 key stages of mammalian cell sub culture | show 🗑
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| What is a protoplast | show 🗑
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| How are different species of plants produced in a lab | show 🗑
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| show | auxin or cytokinins
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| Name a substance a plant needs to grow except plant hormones | show 🗑
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| show | B lymphocyte with a myeloma cell
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| show | injecting mouse with specific antigen that produces antibodies. Removing spleen and extract B lymphocytes & fusing with myeloma cell to form hybridomas
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| What substance are the hybridomas placed in and subsequently diluted to ensure one cell per well | show 🗑
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