202 - Lab 1: Blood Collection
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| show | • Serum/plasma/whole blood
• Animal
• Needle
• Syringe
• Vacutainer
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| WHAT IS AN ANTICOAGULANT? | show 🗑
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| show | NO ADDITIVE
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| LAVENDER TOP BLOOD COLLECTION TUBE | show 🗑
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| show | heparin (sodium or lithium)
o One of the most common tubes
o Not routine in CBC = clumps WBC
o Not used for staining
o Birds, reptiles, fish = RBC have nucleolus so the cell will not lyse
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| show | buffered sodium citrate (just delays the clotting)
o Used for clotting study
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| GRAY TOP BLOOD COLLECTION TUBE | show 🗑
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| show | ACD: Acid Citrate Dextrose
o ACD used all the time when doing blood transfusions
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| show | o Test
o Tube size
o Animal size & hydration status – will effect the volume of blood you can attain
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| MAXIMUM BLOOD COLLECTION SHOULD NOT EXCEED: | show 🗑
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| PCV IS: | show 🗑
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| show | o Jugular vein
o Facial vein
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| show | o Jugular vein
o Coccygeal vein
o Mammary – has a higher natural level of calcium – LAST RESORT
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| OVINE BLOOD COLLECTION SITES | show 🗑
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| CAPRINE BLOOD COLLECTION SITES | show 🗑
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| show | o Ear
o Thoracic Inlet – anterior vena cava
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| show | o Jugular
o Cephalic
o Lateral Saphenous
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| show | o Jugular
o Cephalic
o Medial Saphenous
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| BIRD BLOOD COLLECTION SITES | show 🗑
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| FERRET BLOOD COLLECTION SITES | show 🗑
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| show | lateral saphenous, lateral ear vein (not preferred due to hematoma forming), cephalic vein, not jugular because of dewlap
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| show | JUGULAR
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| RODENT BLOOD COLLECTION SITES | show 🗑
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| EXOTICS BLOOD COLLECTION SITES | show 🗑
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| NEEDLES | show 🗑
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| NEEDLE SIZES | show 🗑
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| SYRINGE SIZES | show 🗑
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| SYRINGE TIPS | show 🗑
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| show | • Three parts:
o Double ended needle
o Plastic sleeve
o Vacuum tube
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| show | • Choose tube, needle, syringe
• Label tube(s)
• Proper restraint
• Locate vein
• Insert needle bevel up
• Draw back on plunger
• Place blood in tube gently – remove top of tube and needle and drip the blood into the tube – this prevents lysis
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Created by:
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