Lab
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| show | The act of preparing & staining diagnostic slides
🗑
|
||||
| show | Examination of cellular material for disease, esp. cancer
🗑
|
||||
| Gynecological cytology specimens | show 🗑
|
||||
| Non-gynecological cytology specimens | show 🗑
|
||||
| Pap smears | show 🗑
|
||||
| ThinPrep System | show 🗑
|
||||
| show | Specimens w/ brush head in vial w/ very weak ethanol preservative,
cell enrichment - centrifuge conc. abnormal cells, remove blood/mucus,
specimen aliquot sediments on slides,
nongynecological req. alt. suspension & centrifugation,
higher yield cells
🗑
|
||||
| show | 95% ethanol,
rapid fixation important to prevent distortion of cell & loss of morphology,
🗑
|
||||
| Alcohol vs. formalin fixation | show 🗑
|
||||
| Other cytology fixatives: | show 🗑
|
||||
| show | Sample w/ not many cells in suspension can be made more conc'd by Cytospin or centrifugation preparation:
1mL properly prepared cell suspension in funnel of cytospin,
centrifuge,
cells will be deposited on slide & fluid absorbed by filter paper
🗑
|
||||
| show | Can obscure morphology or outnumber diagnostic cells,
crosshatch can help separate cells,
dip in Carnoy's or Clark to lyse rbc,
commercial products can lyse rbc (maybe diagnostic too)
🗑
|
||||
| Problem: poorly adhesive specimens | show 🗑
|
||||
| show | Ongoing concern, cells may float into reagents or stick to tools,
change reagents between samples & use disposable tools
🗑
|
||||
| show | Can process, section, stain, as histo section,
easier special stain, incl. IHC,
made of material left after smear,
make from tissue fragments, clots, heavy mucus,
"block" together w/ thrombin-prothrombin clot, albumin, or agar
🗑
|
||||
| show | Smears fixed w/i 1-2 seconds of application to slide,
thick smears/smears w/ mucus may take 2-4 seconds,
liquid suspensions longer,
air-drying causes nuclear swelling, distortion, loss of cytoplasmic density, cytoplasm becomes eosinophilic
🗑
|
||||
| EA stains metabolically inactive cells? | show 🗑
|
||||
| EA stains metabolically active cells? | show 🗑
|
||||
| show | Smear material from scraper/brush in circular motion onto slide in area the size of a nickel,
fix immediately w/ 95% ethanol or spray fixative,
avoid cotton swabs,
avoid feathered-edge smears
🗑
|
||||
| show | 2-3 drops of sediment on slide, place another slide on top upside-down, pull slides apart endways,
fix immediately w/ 95% ethanol or spray fixative,
good general-purpose for fluids w/o mucus
🗑
|
||||
| Crush/mash smear method | show 🗑
|
||||
| show | 2-3 loopfuls of sediment on slide, spread diagonally, back & forth, corner to corner,
fix immediately w/ 95% ethanol or spray fixative,
if sediment beads form, spread again for monolayer of cells,
good for cellular body fluids, esp. w/ lots of blood
🗑
|
||||
| Cytocentrifuge smear method | show 🗑
|
||||
| show | Toluidine blue wet film,
or air-dry & stain w/ Diff-Quik
🗑
|
||||
| Problem: Pap-counterstained smears look dirty & not optimal: | show 🗑
|
||||
| Bodys fluids pleural, peritoneal, pericardial, ascites treatment: | show 🗑
|
||||
| Breast/nipple discharges treatment: | show 🗑
|
||||
| show | Deteriorates rapidly,
if can't take to lab immediately use pre-fixative holding solution - alcoholic saline or Saccomanno fluid = vol. of specimen
🗑
|
||||
| show | Prepare smear at time of collection,
nickel smear,
spray-fix, don't let air-dry
🗑
|
||||
| show | Don't fix, refrigerate immediately
🗑
|
||||
| Urine treatment: | show 🗑
|
||||
| show | Prepare smear at time of collection,
nickel smear,
spray-fix, don't let air-dry,
or submit brush in physiological saline for lab processing,
never fix
🗑
|
||||
| FNAs include: | show 🗑
|
||||
| Alcoholic saline pre-fixative reagents | show 🗑
|
||||
| show | 980 mL ethanol
20 mL melted Carbowax
(remove Carbowax after slide prep, before staining)
🗑
|
||||
| show | Fresh, unfixed specimens, wet-fixed on slide,
better cell adhesion,
causes cells to flatten - better visualization,
pre-fixed specimens - round cells
🗑
|
||||
| show | Scrapings, brushings, viral inclusion smears,
use nickel method
🗑
|
||||
| Fluids include: | show 🗑
|
||||
| show | 2,000 rpm for ~10 min,
high speed packs cells too tightly,
lower speed won't force cells to bottom of tube,
50 mL conical tube best (15 mL usu. not adequate specimen),
swinging-arm centrifuge best concentrates cells @ bottom
🗑
|
||||
| Mucoid specimens include: | show 🗑
|
||||
| Sparsely cellular specimens include: | show 🗑
|
||||
| show | Crush method w/ spray fixative
🗑
|
||||
| Cytopreparation for sample with good cellularity & adhesiveness? | show 🗑
|
||||
| show | Pos slide or cytocentrifuge w/ spray fixative
🗑
|
||||
| show | Cytocentrifuge
🗑
|
||||
| Cell block for specimen w/ spontaneous clots: | show 🗑
|
||||
| show | To remaining sediment add 95% ethanol, denatured alcohol, or 10% NBF, mix by inversion,
fix 15-30 min,
centrifuge 10 min, 2,000 rpm,
pour off supernatant,
mass in cassette, may wrap w/ lens paper,
if large submit portion,
formalin process routinely
🗑
|
||||
| show | To remaining sediment add 30% BSA, mix w/ stick,
add 95% alcohol,
place mass in cassette, may wrap w/ lens paper, if too large submit portion,
formalin process routinely
🗑
|
||||
| show | To remaining sediment add melted 4% agar, mix gently,
centrifuge 10 min, 2,000 rpm,
refrigerate until agar solid,
remove from tube, cut away cellular material, bisect,
place in cassette w/ common cut side down,
formalin process routinely
🗑
|
||||
| Papanicolaou technique stains: | show 🗑
|
||||
| show | Orange G
dH2O
phosphotungstic acid (mordant)
95%ethanol
glacial acetic acid (opt.) (enhance specificity, decr. stain time)
🗑
|
||||
| EA reagents | show 🗑
|
||||
| show | To distinguish cellular components by obtaining highly detailed chromatin, differential counterstaining, & cytoplasmic transparency
🗑
|
||||
| show | hematoxylin
ammonia water, or Scott tap water
OG-6
EA
🗑
|
||||
| show | chromatin - blue
keratin - orange
superficial squamous cells, nucleoli, cilia, rbc - variable pinks
all metabolic cell cytoplasm - variable blue-greens
🗑
|
||||
| show | Rapid determination of cellularity, esp. FNAs,
& eval. potential cross-contamination
🗑
|
||||
| Toluidine blue wet film facts | show 🗑
|
||||
| show | Toluidine blue in 95% alcohol w/ dH2O, filtered
🗑
|
||||
| show | Significant chance of loose cells floating during staining,
to prevent stain separately specimens w/ higher potential to float, filter solutions between batches
🗑
|
||||
| show | Loop electrosurgical excision procedure,
cervix biopsy
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
CCF
Popular Histology sets