Replication Unit for NW 04-350
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| show | hypothetical explanation of how replication might occur. supposes that the original double stranded molecule will remain fully intact after replication is complete and the new double stranded molecule will be entirely composed of new material
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| dispersive model | show 🗑
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| show | hypothetical explanation of how replication might occur. supposes that each strand of the original double stranded molecule remain intact but after replication one strand is paired with one entirely new strand. this hypothesis is supported by data.
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| density gradient centrifugation | show 🗑
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| light nitrogen | show 🗑
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| show | an isotope of nitrogen (N15) with an extra neutron and therefore more mass than light nitrogen
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| origin | show 🗑
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| show | Y shaped structure where the two strands of DNA have separated to serve as templates for replication. each origin will generate two replication forks
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| DNA polymerase | show 🗑
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| show | the prokaryotic form of DNA polymerase that is responsible for synthesizing the majority of the DNA during replication. has higher affinity than other polymerases.
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| show | the more abundant form of DNA polymerase in prokaryotes. has less affinity but can synthesize DNA. can also act as exonuclease in 5' to 3' direction which allows it to remove RNA primers
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| show | the ability of a polymerase to bind to a nucleic acid template and begin synthesizing a complementary strand without anything in place prior to that synthesis. RNA polymerase can do this. DNA polymerases can't
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| single stranded binding proteins (SSB) | show 🗑
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| helicase | show 🗑
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| show | enzyme that moves in front of the helicase, reducing torsional strain on the DNA molecule created when unwinding occurs
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| show | enzyme that synthesizes a small segment of RNA to serve as a primer for initiation of replication.
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| show | needed to provide a 3' OH that DNA polymerases can attach dNTP to when initiating replication. needed because of DNA polymerases inability to carry out de novo synthesis
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| show | strand of new DNA being synthesized in the same direction that the helicase is moving. uninterrupted continuous synthesis until the end is reached
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| show | strand of new DNA being synthesized in the opposite direction that the helicase is moving. goes through periodic starts and stops as new template is exposed behind it
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| Okazaki fragments | show 🗑
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| nick | show 🗑
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| 5' to 3' exonuclease | show 🗑
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| 3' to 5' exonuclease | show 🗑
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Created by:
jthorns
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