ASCP Histology Tech: Processing/Instrumentation
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| show | alcohol, acetone, dioxane, tetrahydrofuran (last two are universal solvents)
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| show | mushy soft sections
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| the dye-check solution of eosin can only be added to ________, not isopropanol | show 🗑
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| __________ cannot be used in processors or with isopropanol | show 🗑
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| clearing agents must be miscible with ______ and _______. | show 🗑
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| show | benzene (clearnat)
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| paraffin used for embedding is ___ degrees celsius. | show 🗑
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| show | it is TOXIC
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| show | it is likely to harden tissue
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| show | 55 to 58 degrees C
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| which is not a clearing agent? chloroform/ethanol/dioxane/xylene | show 🗑
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| show | cracking of the block
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| one advantage of paraffin use is | show 🗑
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| when cutting a block, if the tissue seems hard and brittle... | show 🗑
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| SOFT paraffin is most useful when _______ sections are desired. | show 🗑
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| show | xylene (clearant)
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| show | formation of large crystals
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| show | using water-soluble wax (embedding medium)
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| show | BEST DEHYDRATANT!!!
PEL=1,000 /
60-65-95-95-absolute
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| show | ethanol + methanol or ethanol + isopropanol (dehydratant)
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| METHYL ALCOHOL | show 🗑
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| Dioxane | show 🗑
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| ISOPROPANOL | show 🗑
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| show | (dehydratant) PEL-OSHA=1,000 / PEL-NIOSH=250 / (rapid, cheap, but absorbs water from the air) USED IN TOUCH PREPS OR FAST FIXES. CAN ALSO BE USED LIKE A UNIVERSAL SOLVENT.
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| BUTYL ALCOHOL | show 🗑
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| Tertiary Butanol | show 🗑
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| Tetrahydrofuran | show 🗑
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| show | xylene (clearant)
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| show | BEST HYDROCARBON CLEARING AGENT, PEL=50, STEL=150 /not as hard as xylene
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| show | fast, does not over-harden, WILL HARDEN MUSCLE, TENDON, UTERUS more than TOLUENE // PEL=10
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| XYLENE SUBSTITUTES ARE | show 🗑
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| CHLOROFORM PEL=50 | show 🗑
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| water soluble waxes | show 🗑
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| a nitrocellular compound used for embedding (i. Parloidion). storage blocks held in 80% alcohol. hardened with chloroform. mixed with ether and absolute alcohol to prepare. | show 🗑
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| show | GMA Glycol Methacrylate , acrylic resin which is miscible with water.
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| 5% aqueous formic acid will | show 🗑
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| show | dehydrated
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| show | a dehydratant that retains tissues for extended periods of time. more than ethanol or 70% alcohol
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| sections processed by _______ method show less shrinkage than when processed by paraffin. | show 🗑
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| show | frozen sections
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| show | acid methods
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| these agents EDTA)bind certain metals | show 🗑
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| 30% sucrose | show 🗑
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