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Success: Chemistry Test

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1.
In spectrophotometry, which of the following is a mathematical expression of the relationship between absorbance and transmittance?<br>a. A = <i>abc</i><br>b. A<sub>u</sub>/C<sub>u</sub> = A<sub>s</sub>/C<sub>s</sub><br>c. A = 2 - log %T<br>d. A = log %T<
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2.
Which of the following may be associated with reflectance spectrophotometry as it relates to the dry reagent slide technique?<br>a. Light projected to the slide at 180°-angle<br>b. Dye concentration directly proportional to reflectance<br>c. Unabsorbed, r
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3.
Which of the following is <i>not</i> descriptive of a photomultiplier tube?<br>a. Emits electrons proportionally to initial light absorbed<br>b. Must be shielded from stray light<br>c. Cannot be used with a chopper<br>d. Amplifies the initial signal rec
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4.
Which of the followining is <i>not</i> a problem inherent in turbidimetry?<br>a. Variation in particle size of samples<br>b. Variation in particle size of standards<br>c. Rate of aggregation or settling of particles<br>d. Need to maintain a constant and s
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5.
"Which of the following may be associated with reflectance spectrophotometry as it relates to the dry reagent slide technique?<br>a. Light projected to the slide at 180°-angle<br>b. Dye concentration directly proportional to reflectance<br>c. Unabsorbed
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6.
Which type of photodector employs a linear arrangement that allows it to respond to a specific wavelength resulting in complete UV/visible spectrum analysis?<br>a. Photomultiplier tube<br>b. Phototube<br>c. Barrier layer cell<br>d. Photodiode array<br>
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7.
Fluorometers are designed so that the path of the exciting light is at a right angle to the path of the emitted light. What is the purporse of this design?<br>a. Prevent loss of emitted light<br>b. Prevent loss of the excitation light<br>c. Focus emitted
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8.
Whis of the following isolates light within a narrow region of the spectrum?<br>a. Photomultiplier tube<br>b. Monochromator<br>c. Photovoltaic cell<br>d. Detector<br>
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9.
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the wavelengths that are permitted to impinge on the sample will be within what wavelength range?<br>a. 530-540 nm<br>b. 530-550 nm<br>c. 535-545 nm<br>d. 540-550 nm<br>
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10.
Which of the following may be associated with bioluminescence?<br>a. Light emission produced due to enzymatic oxidation of a substrate<br>b. Less sensitive than direct fluorescent assays<br>c. Electron excitation caused by radiant energy<br>d. Employs a r
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11.
Which of the following best describes chemiluminescence?<br>a. Electron excitation caused by radiant energy<br>b. Enzymatic oxidation of a substrate produces light emission<br>c. Chemical energy excites electrons that emit light upon return to ground stat
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12.
Which of the following formulas is an expression of the Beer-Lambert law that is routinely applied to spectrophotometric analysis?<br>a. A<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = C<sub>u</sub><br>b. C<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = A<sub
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13.
Which of the following may be associated with fluorescence polarization?<br>a. Plane-polarized light is used for sample excitation<br>b. Small molecular complexes show a greater amount of polarization<br>c. It's a heterogenous technique employed in fluo
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14.
Which of the following is <i>false</i> about a photomultiplier<br>a. Converts radiant energy (light) to electrical energy (current)<br>b. Amplifies the current significantly<br>c. Has a very rapid response time<br>d. Is composed of an iron plate and a l
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15.
Which of the following represents a primary advantage of performing fluorometric over absorption spectroscopic methods of analysis?<br>a. Increased specificity and increased sensitivity<br>b. Increased specificity and decreased sensitivity<br>c. Purity of
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16.
In regards to bichromatic analysis, which of the following is <i>false</i>?<br>a. Absorbance is measured at the spectral absorbance peak for a blank and the samples using the same wavelength<br>b. Eliminates background interferences<br>c. Sample concentra
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17.
Which of the following instruments is used in clinical or reference laboratories to detect beta and gamma emissions?<br>a. Fluorometer<br>b. Nephelometer<br>c. Scintillation counter<br>d. Spectrophotometer<br>
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18.
"Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR, and near UV regions of the spectrum?<br>a. Tungsent-filament<br>b. Hydrogen<br>c. Deuterium<br>d. Mercury vapor<br>"
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19.
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass filter used to access?<br>a. Linearity<br>b. Stray light<br>c. Absorbance accuracy<br>d. Wavelength accuracy<br>
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20.
Nephelometry is based on the measurement of light that is:<br>a. Absorbed by particles in suspension<br>b. Scattered by particles in suspension<br>c. Produced by fluorescence<br>d. Produced by excitation of ground-state atoms<br>
A.
Scattered by particles in suspension
B.
Chemical energy excites electrons that emit light upon return to ground state
C.
"Unabsorbed, reflected light detected by photodetector"
D.
Prevent excitation light from reaching the detector
E.
A = 2 - log %T
F.
Plane-polarized light is used for sample excitation
G.
Scintillation counter
H.
Monochromator
I.
Unabsorbed, reflected light detected by photodetector
J.
535-545 nm
K.
Cannot be used with a chopper
L.
A<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = C<sub>u</sub>
M.
Tungsent-filament
N.
Light emission produced due to enzymatic oxidation of a substrate
O.
Is composed of an iron plate and a layer of selenium
P.
Need to maintain a constant and specific temperature
Q.
Photodiode array
R.
Increased specificity and increased sensitivity
S.
Absorbance is measured at the spectral absorbance peak for a blank and the samples using the same wavelength
T.
Wavelength accuracy
Type the Answer that corresponds to the displayed Question.
incorrect
21.
Which of the following isolates light within a narrow region of the spectrum?<br>a. Photomultiplier tube<br>b. Monochromator<br>c. Photovoltaic cell<br>d. Detector<br>
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22.
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the wavelengths that are permitted to impinge on the sample will be within what wavelength range?<br>a. 530-540 nm<br>b. 530-550 nm<br>c. 535-545 nm<br>d. 540-550 nm<br>
incorrect
23.
Which of the following instruments is used in clinical or reference laboratories to detect beta and gamma emissions?<br>a. Fluorometer<br>b. Nephelometer<br>c. Scintillation counter<br>d. Spectrophotometer<br>
incorrect
24.
Which type of photodector employs a linear arrangement that allows it to respond to a specific wavelength resulting in complete UV/visible spectrum analysis?<br>a. Photomultiplier tube<br>b. Phototube<br>c. Barrier layer cell<br>d. Photodiode array<br>
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25.
Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR, and near UV regions of the spectrum?<br>a. Tungsent-filament<br>b. Hydrogen<br>c. Deuterium<br>d. Mercury vapor<br>
incorrect
26.
"When performing spectrophotometer quality assurance checks, what is the holmium oxide glass filter used to access?<br>a. Linearity<br>b. Stray light<br>c. Absorbance accuracy<br>d. Wavelength accuracy<br>"
incorrect
27.
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass filter used to access?<br>a. Linearity<br>b. Stray light<br>c. Absorbance accuracy<br>d. Wavelength accuracy<br>
incorrect
28.
Which type of photodector employs a linear arrangement that allows it to respond to a specific wavelength resulting in complete UV/visible spectrum analysis?<br>a. Photomultiplier tube<br>b. Phototube<br>c. Barrier layer cell<br>d. Photodiode array<br>
incorrect
29.
Which of the following instruments is used in clinical or reference laboratories to detect beta and gamma emissions?<br>a. Fluorometer<br>b. Nephelometer<br>c. Scintillation counter<br>d. Spectrophotometer<br>
Type the Answer that corresponds to the displayed .
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30.
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