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Order Of Draw
Phlebotomy OOD
Question | Answer |
---|---|
What are the Yellow tube (blood culture) additives? | Acid, Citrate, Dextrose (ACD) AND Sodium Polyanethle Sulfonate (for blood cultures) |
What are the Yellow tube (blood culture) Lab Uses? | Blood Bank Studies |
What is the Yellow tube (blood culture) specimen types? | Whole Blood |
What are the Yellow tube (blood culture) Clinical Correlations? | Sepsis and Infection |
What are the Yellow tube (blood culture) number of inversions? | 8 |
What is the Yellow tube (blood culture) rationale for collection order? | Minimizes the chance of microbial contamination |
What is the Yellow tube (blood culture) Draw volume? and what is it typically drawn into? | 8.0 mL (typically drawn into a culture bottle) |
What are the Red tube Additives for glass? | it is an SST |
What are the red tubes Additives for plastic? | it has a clot activator |
What is the Red tube mode of action? | Blood clots, and serum separates by cetrifugation |
What are the Red tube Lab Uses? | Chemistry , serology, Immunology, and blood bank |
In the Red tube, what tests are done in the chemistry lab use? | glucose, cholesterol, triglycerides, HDL, potassium, amylase, PSA, BUN, liver enzymes, C-reactive protein |
In the Red tube, what tests are done in the serology lab use? | antibody to Hep B, RPR for syphilis, drug screening, SLE, Vitamins A&E, Monospot, Rheumatoid factor, ANA |
In the Red tube, what tests are done in the immunology lab use? | Antistreptolysin O (ASO), Rubella titer, inflammatory disease |
In the Red tube, what tests are done in the blood bank lab use? | antibody screen, direct antihuman globulin test (DAT), |
What is the Red tubes number of inversions? | 5 |
What is the Red tubes draw volume? | 2.0 mL- 6.0 mL |
What drugs can you test for with the Red tube? | Hydrocodone, Prednisone, Lorazepam, Trazodone |
What is the Red tubes specimen type? | Serum |
What is the Red tubes clinical correlation? | Coronary risk, STI determination, autoimmune diseases |
What are the Additives in the Light Blue GLASS tube? | Sodium Citrate 3.2% |
What are the Additives in the Light Blue PLASTIC tube? | Sodium citrate 3.8% |
What is the lab use for the Light Blue tube? | Coagulation |
What does Coagulation test for in the light blue tube? | Anti-thrombin III, Platelet count, BT, PT, PTT, APTT, Fibrinogen |
How many inversions for the Light Blue tube? | 3-4 inversions |
What is the draw volume for the light blue tube? | 9-1 ratio; Fill and Chill; FULL DRAW (3.5 mL) |
What type of specimen is used for the Light blue tube? | Plasma |
What is the Clinical correlation on the Light Blue tube? | Coagulation Disorders, platelet function, heparin/Coumadin therapy |
What is the rationale for the collection order in the light blue tube? | minimizes the change of microbial contamination |
What are the additives for the Gold top tube? | Clot activator, and serum separator gel |
what are the lab uses for the gold top tube? | Chemistry, blood bank, serology, immunology |
In the Gold tube, what tests are done in the chemistry lab use? | Chemistry profile, serum determination in chemistry, bilirubin, potassium, triglycerides, cholesterol, lipid panels, MMR, PSA, TSH, Endocrine studies |
In the Gold tube, what tests are done in the Blood Bank lab use? | Antibody screen, ABO and Rh typing |
In the Gold tube, what tests are done in the serology lab use? | HIV, Monospot, HCG |
In the Gold tube, what tests are done in the immunology lab use? | Epstein-Barr, Hep B&C, Herpes simplex |
What is the Rationale for the collection order on the gold tube? | Comes after coag tube, and before any therapeutic drug testing |
How many inversions for the gold tube? | 5 |
what is the draw volume for the gold tube? | 2.0 mL- 6.0 mL |
What is the Mode of action for the gold SST tube? | the gel at the bottom of the tube separates the serum from the blood when being centrifuged |
What drugs can be tested for in the Gold tube? | Levothyroxine, and Synthroid |
What type is specimen is used for the gold tube? | Serum |
What is the clinical correlation of the gold tube? | Coronary risk, autoimmune diseases, blood grouping (ABO & Rh), and endocrine studies |
What are the additives for the Tiger top tube? | Clot Activator, and Serum Separator gell |
What are the Lab Uses for the tiger top tube? | Chemistry, Blood Bank, Serology, immunology |
In the tiger top tube, what tests are done in the chemistry lab use? | Glucose, Cholesterol, triglycerides, CRP, potassium, dx testing, rubella, Fe, Drug screens |
In the tiger top tube, what tests are done in the serology lab use? | Anti HIV, HCG |
In the tiger top tube, what tests are done in the immunology lab use? | Febrile Agglutinins |
In the tiger top tube, what tests are done in the Blood Bank lab use? | type & cross match, type and screen |
What is the Rationale for the collection order on the gold tube? | Comes after coag tube, and before any therapeutic drug testing |
How many inversions for the tiger top tube? | 5 |
What is the draw volume for the tiger top tube? | 4.0 mL |
What drugs can be tested for in the Tiger Top tube? | Simvastatin, Lipitor, Metroprolol, Furosemide, metroprolol tartrate, Plavix, Lovastin |
What is the type of specimen for the tiger top tube? | Serum |
What is the clinical correlation for the tiger top tube? | Coronary risk, STI's, infection, and blood typing |
What are the additives for the Light Green tube? | Lithium Heparin, and Plasma separation gel |
what is the lab use for the light green tube? | Chemistry |
What tests are being performed in the chemistry lab use of the light green tube? | renal function testing, hepatic panel, TSH, BUN, Uric acid, Lipid Panel, Plasma determination, potassium determination |
What is the mode of action for the light green tube? | Anticoagulate with lithium heparin; plasma is separated with the separating gel at the bottom of the tube |
what is the rationale for the collection order of the light green tube? | chemistry testing generally comes after coag and or serum testing |
how many inversion for the light green tube? | 8 |
what is the draw volume for the light green tube? | 3.5 mL |
what is the specimen type for the light green tube? | plasma |
what is the clinical correlation for the light green tube? | Kidney function, endocrine function, and liver function |
what are the additives for the green/gray tube? | lithium heparin, and Plasma separation gel |
what are the lab uses for the green/gray tube? | Chemistry |
what tests are in the chemistry lab use for green/gray tube? | ABG, Plasma determination, potassium determination, STAT chemistry tests |
what is the rationale for the collection order for the green/gray tube? | chemistry testing generally comes after coag and or serum testing |
how many inversions for the green/gray tube? | 8 |
what is the draw volume for the green/gray tube? | 2.0 mL |
what is the clinical correlation for the green/gray tube? | Chemistry studies |
What are the additives for the Kelly Green tube? | lithium heparin and sodium heparin |
what is the lab use for the kelly green tube? | cytogenic testing |
what is the rationale for the collection order for the Kelly green tube? | chemistry testing generally comes after coag and or serum testing |
how many inversions and what id the draw volume for the kelly green tube? | 5 inversion and 4.0 mL |
what type of specimen is the kelly green tube? | plasma |
what is the clincal correclation for the kelly green tube? | genetic testing |
what are the additives of the dark green tube? | lithium/sodium/ammonium heparin |
what is the dark green tubes mode of action? | inactivates thrombin and thromboplastin |
What is the lab use for the dark green tube? | Chemistry |
What lab tests can be done in chemistry for dark green tube? | Dilantin, phenobarbital, plasma determination, STAT electrolytes, pH, FSH, Chromosome studies, Vitamin C, ammonia, calcium, cortisol, thiamin, potassium |
what is the rationale for the collection order for the dark green tube? | chemistry testing generally comes after coag and or serum testing |
how many inversions and what is the draw volume for the dark green tube? | 8 inversion; 4.0 mL |
what drugs can be tested with the dark green tube? | hydrochlorothyazide, ibuprofen, lisinopril, triamterene, Diovan, Crestor, warfarin |
what is the specimen for the dark green tube? | Plasma |
What is the Clinical correlation for the dark green tube? | Endocrine studies, chromosome studies, clotting factor, mineral and hormone testing |
What is the additive for the white top? | potassium K2EDTA (with gel) |
What is the lab use for the white top? | Chemistry |
what tests in chemistry are ran on a white tube? | DNA testing, Molecular dx testing, Polymerase Chain reaction |
What is the rationale for the collection order on the white tube? | follows plasma tests and does not contain heparin |
What is the mode of action on the white tube? | Forms Calcium salts |
how many inversions and what is the draw volume on the white tube? | 8 inversions; 4.0 mL |
Specimen type for the white top? | Whole Blood/plasma |
Clinical correlation for the white top? | DNA testing |
what are the additives in the plastic pink top? | spray dried K2EDTA |
what is the additive in the glass pink top? | potassium K3EDTA |
what is the lab use for the pink tube? | Blood bank |
what tests are done in the pink tube for the blood bank lab use? | whole blood hematology, blood banking, routine immunohematology testing, blood donor screening |
what is the mode of action for the pink tube? | forms calcium salts |
what is the rationale on the collection order for the pink tube? | follows plasma tests and does not contain heparin |
how many inversions and what is the draw volume on the pink tube? | 8 inversions; 6.0 mL |
what is the specimen type in the pink tube? | whole blood/plasma |
what is the clinical correlation in the pink tube? | hematology, immunity testing, blood banking |
What are the additives in the Glass lavender tube? | liquid K3EDTA |
What are the additives in the plastic lavender tube? | K2EDTA |
what is the mode of action in the lavender tube? | forms calcium salts to remove calcium salts |
what are the lab uses in the lavender tube? | blood bank, and hematology |
what are the tests in the blood bank lab use for the lavender tube? | CBC, blood bank, HIV, Rh, Vitamin D, A1C |
what are the tests in the hematology lab use for the lavender tube? | platelet function, Hgb, Hct |
Rationale for the collection order for the lavender tube? | Follows pink for hematology |
how many inversions and what is the draw volume for the lavender tube? | 8 inversions and 4.0 mL |
what is the specimen type for the lavender tube? | whole blood/ plasma |
what is the clinical correlation for the lavender tube? | anemia, bleeding disorders, ABO, glucose testing, clotting capabilities |
what are the additives for the gray top? | NA2, potassium oxalate, sodium fluoride |
what is the mode of action for the gray top? | anti-glycotlytic agent preserves glucose up to 5 days |
what are the chemistry lab uses for the gray top? | glucose testing, FBS, GTT, postprandial, lactic acid, ETOH (with witness) Cocaine Testing |
Gray tubes reason for collection order is? | Fluoride destroys enzymes, so it follows hematology tests |
how many inversions and what is the draw volume for the gray tube? | 8 inversions; 4.0 mL |
what type of specimen is the gray tube? | Whole blood |
what is the clinical correlation in the gray tube? | Glucose testing, alcohol testing, cocaine testing |
Yellow tubes (number 14) additives | ACD, sodium polyanethol sulfonate |
what is the yellow tubes mode of action when number 14 in OOD | compliments inactivation |
when the yellow top is #14 in OOD the lab use is for what tests? | HLA, paternity testing, DNA studies, rape studies, cellular studies |
how many inversions and what is the draw volume for the yellow tube when it is #14? | 8 inversions; 3.0 mL |
what specimen type is the yellow tube in #14 OOD? | Whole Blood |
What is the clinical correlation in the yellow tube when it is #14 in the OOD? | Paternity testing, DNA and cellular studies |
What are the additives for the orange top? | thrombin |
what are the lab uses for the orange tube? | Chemistry and microbiology |
what tests are ran for the chemistry lab use in the orange tube? | STAT serum determination, anticoag therapy, autoantibodies |
what tests are ran for the microbiology lab use in the orange tube? | microbial, parasitic, and viral studies |
what is the rationale for the collection order for the orange tube? | additive tubes follow non additive and coag tubes |
what is the mode of action for the orange tube? | blood quickly clots |
how many inversions and what is the draw volume for the orange tube? | 8 inversions; 3.0 mL |
what is the specimen type for the orange tube? | whole blood/ serum |
what is the clinical correlation for the orange tube? | coagulation, parasitic, and viral studies |
What are the additives for the dark blue tube? | NA2EDTA clot activator (plastic for serum), sodium heparin |
what are the lab uses for the dark blue tube? | chemistry |
what chemistry tests are used for the dark blue tube? | nutritional studies, trace element testing, toxicology |
what is the rationale for the collection order for the dark blue tube? | additive tubes follow non additive and coag tubes |
what is the mode of action for the dark blue tube? | tube is designed to contain no contaminating metals |
how many inversions and what is the draw volume for the dark blue tube? | 8 inversions; 3.0 mL |
what type of specimen is the dark blue tube? | plasma |
what are the additives for the brown/tan tube? | sodium heparin, spray dried K2EDTA (plastic) |
what is the chemistry lab use for the dark blue tube? | Lead Determination |
what is the rationale for the collection order for the brown/tan tube? | additive tubes follow non additive and coag tubes |
how many inversions and what is the draw volume of the brown/tan tube? | 8 inversions; 6.0 mL |
Specimen type of the brown/tan tube? | plasma |
what are the additives for the yellow/black tube? | broth mixture |
the lab use for the yellow/black tube is microbiology, what tests are done in this type? | aerobes, anaerobes, fungi |
what is the rationale for the collection order for the yellow/black tube? | additive tubes follow non additive and coag tubes |
how many inversions and what is the draw volume for the yellow/black tube? | 6-8 inversions; FULL DRAW |
What is the specimen type for the yellow/black tube? | whole blood |
what is the additive for the black tube? | sodium citrate (buffered) |
what are the lab tests for hematology in the black tube? | ESR, Westergren sedimenatation rate |
what is the rationale for collection order for the black tube? | additive tubes follow non additive and coag tubes |
how many inversions and what is the draw volume for the black tube? | 8 inversions; FULL DRAW |
what is the specimen type for the black tube? | whole blood |
what are the additives for the clear tube? | there are none |
what is the lab use for the clear tube? | discard tube or secondary specimen tube |
rationale for collection order for the clear tube | use PRN |
inversions and the draw volume for the clear tube? | N/A; Use PRN |
specimen type for the clear tube? | Varies |