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UND 363 Thio T
UND 363 Thioflavin T
| Question | Answer |
|---|---|
| purpose of Thioflavin T flourescent method | demonstrate Amyloid (not as specific as congo red) |
| principle of Thioflavin T | Thioflavin T attaches to amyloid, alum hematox will reduce the background/nuclear flourescence |
| that will be seen with Thio T | Good visualization of even minimal deposits, but other components can be shown (light up) as well (keratin, paneth cells) |
| fixatives of Thioflavin T | 10% NBF |
| thickness/control | 6-10 microns, control must have known positive for amyloid |
| microscope for of Thioflavin T | flourescent |
| name the 3 major reagents of Thioflavin T | Mayer hematox - reduce nuclear flourescence thioflavin t - flourescent amyloid stain acetic acid - differentiation step |
| what type of mounting media must be avoid and give an example | must avoid non flourescent mount media canada balsam media will autoflouresce |
| how can the thio T stain be mounted directly from water | it can be mounted from water if the apathy mounting media is used |
| what will occur to thioflavin t in sunlight | thioflavin T will detiorate quickly if kept in sunlight **also slides held for long time will deteriorate if held for long time** |
| typical results of Thioflavin T | amyloid - flouresces yellow to yellow green |
| what ph is recommended for Thio T | PH 1.4 |
| What can be used to give a brighter yellow flouresce | using an exciter filter UG1 and colorless UV barrier filter |
| why is ph important for thioflavin T | it will increase selectivity of the dye for amyloid |
Created by:
mustangvxd
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