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UND 363 Verhof Elast
UND 363 Verhoeff Elastic
| Question | Answer |
|---|---|
| Purpose for verhoeff elastic | demonstrate elastic fibers and demo. atrophy of elastic tissue |
| principle for verhoeff elastic | overstained w/ regressive heme, diff'd by excess mordant, sodium thiosulfate removes excess iodine, then counterstained with van geison or "other soln'" **elastic tissue has affinity for iron heme and will retain dye longer than other elements** |
| what makes up the regressive hematox | iron hematoxylin/ferric chloride/iodine |
| what mordant diff's the stain | ferric chloride breaks tissue-mordant dye complex (ie dye is attracted to excess mordant and is removed) |
| for verhoeff elastic principle what part does the elastic tissue have | has an affinity for iron hematox and will retain dye longer than other elements (ie overstained and then diff'd back) |
| fix for verhoeff elastic | 10% NBF or zenkers |
| microns for | 4-5 microns |
| Quality control | artery in CROSS SECTION and embedded ON EDGE |
| Reagents for verhoeff elastic | Verhoeff's hematox overstain (lake of hemeatox, ferric chloride, iodine) - stain nuclei and elastic, Ferric chloride/lugols iodine (1st a mordant, 2nd oxidize hematox to hemetein, van geison - counterstain, soduim thiosulfate - removes excessive iodine |
| typical results for verhoeff elastic | Elastic - blue/black to black, Nuclei blue to black, Collagen - Red, Other tissues - yellow |
| issues with over diff'ing | easily done, if over diff'd restain (but can only be done if NOT treated with alcohol) |
| what will occur if staining prolonged in van geison | the picric acid will diff the stain further |
| why is saturated picric acid a need | collagen will not stain red and cyto/muscle/collage, will stain the same color |
| how must the elastic stain be prepared | the reagents must be added in order and mixed after each addition or the staining may be poor |
| stain jar for verhoeff elastic can be cleaned how | transfer the 2% ferric chloride to jar for a few min. and discard |
| what is a helpful hint for getting proper diff's | use duplicate sections diff'd to slightly different end point |
| since the staining is dependant on amt. of elastic tissue what is the optimal method | slides should be individually diff'd |
| if mercury deposits are seen what should be done | nothing, they will be removed with the staining solution. |
Created by:
mustangvxd
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