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UND 363 Osm. Tet
UND 363 Osmium Tet. Paraffin
| Question | Answer |
|---|---|
| purpose for osmium tetroxide paraffin proc. | demo of fat that allow for paraffin embedding of tissue |
| principle for osmium tetroxide paraffin proc. | osmium tet. combines with fat as a reductioning compound, blackening it. Fat combined with osm. tet. is insoluble in alcohol and xylene and can be processed for paraffin embedding |
| fix for osmium tetroxide paraffin proc. | 10% NBF |
| tech | stain wet tissue block, then process in tissue processor, starting at 70% alcohol. (section should be no thicker than 2mm or osm tet will not penetrate (penetrates slowly) |
| QC | no control need, most tissue contains fat |
| procedure | trim 10% nbf tissue to 2mm, put in osm. tet. (time depends on tissue and size), diff with periodic acid, process routinely starting in 70% alcohol, cut at 4-5 microns, stain with H&E |
| what can be used as a counterstain for this procedure | massons trichrome |
| results | fat - black, other tissue (depends on method) |
| what amounts of fat can be fixed with this method | only small droplets or individual fat cells, gross amounts of fat will not fix |
| how should facing be done | faced carefully, sections taken as soon as possible. osm. tet. doesn't penetrate far and interior of block may not have good preservation or stain well |
| how will the cytoplasm stain | will be grey because of osm. tet. |
Created by:
mustangvxd
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