Save
Busy. Please wait.
Log in with Clever
or
show password
Forgot Password?
Don't have an account?  Sign up 
Sign up using Clever
or
Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account. Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't Know
Remaining cards (0)
Know
0:00
edit
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 HotchkissPAS

UND 363 Hotchkiss PAS

QuestionAnswer
principle for Hotchkiss PAS demo of fungi
principle for Hotchkiss PAS polysaccharides in cell walls are oxidized with periodic acid to aldehydes. The aldehydes react with Schiff to create a rose colored fungi
fix NBF, bouin, zenker
micron 4-5
QC section with fungi
reagents in order periodic acid - oxidizer, schiff reagent - react with aldehys for pink color, sulforous rinse - remove excess schiff and prevent false colorization, fast green 1:5000 - counterstain
results fungi - rose, background - green
if there is a lack of contrast and weak background what is the issue and what can be done? the oxidizing soln' (periodic acid) is weak therefore it will not oxidize native carbs past the aldehyde stage which in turn allows these elements and carb rich fungus to react with schiff, FIX - use strong oxidizer (ie chromic acid)
what is another reason there is a lack of contrast and weak background what is the issue and what can be done? the schiff reagent is old and depleated, FIX - use fresh
if there is weak staining/fungus not staining what can the problems be and the FIX Exhausted oxidizer - FIX use fresh, Overused schiff reagent will produce poor results - FIX use fresh
Created by: mustangvxd
Popular Histology sets

 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!
"Know" box contains:
Time elapsed:
Retries:
restart all cards