Save
Busy. Please wait.
Log in with Clever
or
show password
Forgot Password?
Don't have an account?  Sign up 
Sign up using Clever
or
Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account. Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't Know
Remaining cards (0)
Know
0:00
edit
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Gridley

QuestionAnswer
purpose for Gridley Fungus Stain demo fungi
principle chromic acid oxidizes adjacent glycol groups to aldehydes. Aldehydes then react to schiff reagent.
how is this principle different than the PAS stain chromic acid is a stronger oxidizer than periodic acid and will destroy/turn some aldehydes to acid therefore leaving less reactive groups to react with schiff. (less intense reaction than PAS but also less background staining.
In addition to the chromic acid destroying many aldehyde groups what will the aldehyde fuchsin do in its place. the aldehyde fuchsin will act as an aldehyde and occupy uninvolved linkages of the schiff reaget thereby reinforcing the depth of the stain.
fix NBF
micron 4-5
QC section with fungus
reagents in order 4% chromic acid - oxidizer, Schiff - reacts with aldehydes, aldehyde fuchsin - acts as aldehyde by occupying uninvolved linkages of schiff reagent, metanil yellow - counterstain
results mycelia/elastic fibers/mucin - deep purple, conidia - deep rose to purple, background - yellow
how will old non-viable fungi be stained with this technique? they are not well stained if they are non-viable
what type of acid can be used after the schiff reagent sulfurous acid - will remove excess schiff reagent
of pararosaniline (CI42500) and basic fuchsin (rosaniline CI 42510) which is use to prepar the aldehyde fuchsin pararosaniline
explain in detail what the chromic acid will do the glycols (in trace amts. Vs. those with heavy polysaccharide %) oxidizes 1,2 glycols to aldehydes and then oxidizes some further to acid (trace amts.) Tissue with heavy polysaccharides (mucin, glycoten, fungi) take longer and will still show + PAS RXN even after basement membrane and conn. tissue are non-reactive.
Created by: mustangvxd
Popular Histology sets

 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!
"Know" box contains:
Time elapsed:
Retries:
restart all cards