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UND 363 Alc Blu 2.5
UND 363 Alcian Blue 2.5
Question | Answer |
---|---|
purpose for Alcian Blue 2.5 | demo mucoid capsule of cryptococcus |
principle for Alcian Blue 2.5 (cryptococcus) | alcian blue color due to copper, used in 3% acetic acid A.B stains sulfated and carboxylated acid mucopolysaccharides. A.B forms salt linkages with acid groups of acid mucopolysaccharides |
fix for Alcian Blue 2.5 (cryptococcus) | NBF or bouins |
micron for Alcian Blue 2.5 (cryptococcus) | 4-5 |
Qc | section with know cryptococcus |
reagents in order for Alcian Blue 2.5 (cryptococcus) | pre 3% acetic acid - protect PH, alcian blue - stain mucin, post 3% acetic acid - prevents nonspecific staining, nuclear fast red - counterstain |
results | cryptococcus - pale blue, background - pink to red |