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Central Dogma
Some good Stuff
Term | Definition |
---|---|
Where and when during the cell cycle does DNA replication occur? | In the nucleus during the "S" part of inter-phase. |
Exactly what gets replicated during DNA replication? | The 46 chromosomes in the nucleus, every single one of them gets copied the entire library. |
Adenine bonds with _______ Cytosine bonds with ______ | Thymine Guanine |
Semi-conservative Replication | Occurs the two DNA molecules twist into TWO double helices. Each strand is half old and half new. |
Helicase | Enzyme that unwinds the helix, and separates the two strands. This action requires ATP. |
Gyrase (topoisomerase) | prevents tangling of the DNA as it unwinds |
RNA Primase | A special RNA polymerase that that makes short RNA primers that allow DNA polymerase to work. A primer is required because there must be a free 3' OH group available for the addition of each nucleotide. |
DNA Polymerase III | Recognizes the RNA primer and Synthesizes DNA in the 5'->3' direction.(DNA synthase always occurs in the 5'->3' direction) |
DNA Polymerase I | Removes the RNA primers and replaces them with DNA. |
DNA Ligase (Glue Enzyme) | Makes sure that the newly-synthesized DNA is covalently-linked strand. |
Leading Strand | The DNA strand that is synthesised continuously during replication. |
Lagging Strand | The strand of DNA whose direction of synthesis is opposite to the direction of the growing replication fork, and is much more complex. |
Okazaki Fragments | Short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication. They are complementary to the lagging template strand, together forming short double-stranded DNA sections. |
The Central Dogma of Molecular Biology | DNA makes RNA, RNA makes proteins, and proteins make traits. |
How does DNA control the cell | By telling the cell what proteins to make. |
The three types of RNA | -mRNA -tRNA -rRNA |
What does protein synthesis make sure of? | It makes sure you can form the same protein the same way every time. |
Where does Protein Synthesis occur? | The first part occurs in the nucleus, and the second part occurs in the Cytoplasm. |
When does Protein Synthesis occur? | It occurs on an as needed basis, or only when you need that protein. |
What gets replicated during Protein Synthesis? | 1 gene on one chromosome (this is where the recipe for that protein is located. |
What happens during Transcription? | The DNA recipe for a protein get copied down or translated into RNA language. |
What occurs during translation? (simplified) | The RNA message gets copied down in amino acid language, and the protein gets assembled. |
What is the tRNA carrying on its other end? | The tRNA is carrying a specific amino acid. |
What does a ribosome call for in Translation | The tRNA with the correct anticodon to match that codon. |
Codon | Made up of three nitrogenous bases, every three bases is one. AUG|CCC|GAU|GUU etc. |
What is the only start codon. | AUG or methionine. |
Peptide Bond | The bond formed by amino acids. |
Single-Strand Binding proteins | Bind to the sugar phosphate backbone of the DNA molecule so that it can't bind back together |