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Histology: Lab

Histology ASCP: Lab protocol and safety

QuestionAnswer
Removal of water Dehydration
List dehydrants Ethyl alcohol, Methyl alcohol, Isopropyl alcohol, Butyl Alcohol, Acetone
Reagents that perform both the dehydrating and clearing steps. Universal solvents
List universal solvents Dioxane, Tertiary Butanol, Tetrahydrofuran
Have high index of refraction and will render tissue transparent Clearing agent
Sometimes referred to as dealcoholization agents Clearing agent
List clearing agents Xylene, Toluene, Benzene, Chloroform, Acetone, Essential oils, Limonene (Xylene substitute), Aliphatic Hydrocarbons (Xylene substitute)
Holds the cells and intercellular structures in their proper relationship while thin sections are cut Infiltrating medium
List infiltrating medium Paraffin, Water-Soluble Waxes (Carbowax), Celloidin, Plastics (Glycol Methacrylate and Epoxy Resins), Agar and Gelatin, 30% Sucrose
Better support for hard tissue Higher melting point
Easier to obtain thin sections Higher melting point
Ribboning becomes more difficult Higher Melting point
Poor support for hard tissue Lower melting point
Thin sections are more difficult to obtain Lower Melting Point
Ribboning becomes easier Lower Melting Point
Organic compounds that have the property of binding certain metals Chelating agents
Occulars, or eyepieces 10x magnification
High powered dry lens 40x to 45x magnification
Scanning lens 2.5x to 4x magnification
Intermediate lens 10x to 20x magnification
Oil immersion lens 90x to 100x magnification
Enlarging an object without revealing any increased detail Empty magnification
Using to examine tissue for substances exhibiting the phenomena of double refraction, anisotropism and birefringence Polarizing microscope
Used for the examination of unstained specimens, especially unstained living cells, and allows almost transparent objects to be seen clearly. Phase-Contrast Microscope
Directly transmitted light is excluded and only scattered or oblique light is used Darkfield Microscopy
Used primarily for the study of unstained microorganisms and for silver grains in radioactive staining procedures. Darkfield Microscope
Optical phenomenon in which light of one wavelength is absorbed by a substance and almost instantly re-emitted as a light of a longer wavelength. Flourescence
The specimen either transmits electrons or deflects electrons. A 2D black and white image is seen. Transmission Electron Microscope
A 3D image results as the electron beam sweeps the surface of the specimen and releases secondary electrons. Scanning Electron Microscope
Result when horizontal edges of the block are not parallel Crooked ribbons
Occurs when block is faced too aggressively Holes in the section
Caused by too little blade tilt Lifting of section from the blade, skipped sections
Occurs in hard tissue or overfixed tissue Washboarding or undulations
Occurs in overdehydrated tissue, or by too much blade tilt Microscopic chatter
In Cryostat sections there is a natural tendency for curling and rolling of sections that can be reduced by a: Antiroll plate
In Cryostat sections if a particular tissue tends to split The temperature may be too low
In Cryostat sections if the sections tend to collect at the blade edge The temperature may be too warm
Cryostats are operated at appoximately -20 degree Celsius for most tissues
In Cryostat sections: Brain, liver,spleen, lymph node and endometrial scrapings section better at: Warmer temperatures
In Cryostat sections: fat requires A much colder temperature
Fires involving ordinary combustible materials such as wood, plastics, paper and textiles Class A Fires
Can be extinguished with water or water-based solutions Class A Fires
Fires involving flammable liquids and gases Class B Fires
Requires oxygen to be blocked from the fuel in order to be extinguished Class B Fires
Electrical Fires Class C Fires
Must be extinguished with nonconductive media Class C Fires
Fires of combustible and reactive elements, such as metallic sodium, potassium, magnesium and lithium. Class D Fires
Difficult to control and extinguish because spreading and explosion can occur easily Class D Fires
Health rating Left Blue Diamond
Flammability hazard Top Right Diamond
Reactivity Right Yellow Diamond
Contains special symbols indicating properties and categories Bottom White Diamond
Created by: impossiblesum
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