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Nerve
Nerve stains in the histology lab
| Question | Answer |
|---|---|
| What are the two parts of the nervous system? | Central and peripheral |
| CNS | Spinal cord and brain |
| Somatic nervous system | Voluntary (conscious control) |
| Autonomic nervous system | Involuntary |
| Four types of glial cells | 1. Oligodendroglia 2. Astrocytes 3. Microglia 4. Ependymal cells |
| Myelin consists of | protein, chloesterol, phospholipids, and cerebrosides |
| Crsyl echt violet method I | To identify neurons in tissue sections or the demonstration of the loss of Nissl substance |
| Crsyl echt violet method I | Neurons contain nissl substance, which is primarily composed of rough endoplasmic reticulum. Nissl substance is basophilic and stains well with aniline dyes. |
| Crsyl echt violet method I | 10% NBF |
| Crsyl echt violet method I | 6-8 micrometers |
| Crsyl echt violet method I control | Spinal cord |
| Crsyl echt violet method I | 1.Crsyl violet 2. Rinse 3. 95% alcohol 4. 100% alcohol 5. xylene 6. balsam-xlene 7. 100% alcohol 8. Xylene (Steps 6-8 will have to be repeated till proper differentiation is reached) |
| Crsyl echt violet method I results | Nissl substance: Blue to purple Nuclei: Blue to purple Background: Colorless |
| Creysl echt violet method II | Identify neurons in tissue sections or the demonstration of loss of Nissl substance (chromatolysis) |
| Creysl echt violet method II | 10% NBF |
| Creysl echt violet method II | 6-8 micrometers |
| Creysl echt violet method II control | spinal cord |
| Creysl echt violet method II | 1. Cresyl echt violet 2. Dehydrate 3. Clear |
| Creysl echt violet method II | Nissl substance and nuclei: Blue-purple Background: Colorless |
| Bodian method | Nerve fibers, nerve endings, neurofibrils |
| Wallerian degeneration | When an axon is severely or irreversibly injured, all of the axon distal to the injury disappears along with its myelin sheath |
| Bodian method | 10% NBF |
| Bodian method | 6-8 micrometers |
| Bodian method control | Peripheral nerve or cerebral cortex |
| Bodian method | 1. Protargol 2. rinse 3. reducing solution 4. rinse 5. gold chloride 6. rinse 7. Oxalic acid 8. rinse 9. sodium thiosulfate 10. rinse 11. counterstain with aniline blue 12. dehydrate 13. clear |
| Bodian method results | Nerve fibers: Black Background: Light gray or blue Nuclei: Black |
| Holmes silver nitrate method | Demonstration of Nerve fibers and neurofibrils |
| Holmes silver nitrate method | 10% NBF |
| Holmes silver nitrate method | 10-15 micrometers |
| Holmes silver nitrate method control | Cerebral cortex |
| Holmes silver nitrate method | 1. 20% silver nitrate 2. rinse 3. impregnating solution 4. reduce 5. wash 6. gold chloride 7. rinse 8. 2% Oxalic acid 9. rinse 10. 5% sodium thiosulfate 11. wash 12. dehydrate 13. clear |
| Holmes silver nitrate method results | Axons and nerve fibers: Black Neurofibrils: Black |
| Bielschowsky-PAS | Nerve fibers, neurofibrillary tangles, and senile plaques |
| Bielschowsky-PAS | Diagnoses Alzheimer |
| Bielschowsky-PAS | 10% NBF |
| Bielschowsky-PAS | 8-10 micrometers |
| Bielschowsky-PAS control | CNS that contains senile plaques and neurofibrillary tangles |
| Bielschowsky-PAS | 1. 20% silver nitrate 2. w 3. Ammonical silver solutions 4. w in ammonia water 5. Add developer 6. w 7. gold chloride 8. Ammonia water 9. 5% sodium thiosulfate 10. w 11. 1% periodic acid 12. wash 13. Schiff 14. wash 15. Dehydrate and clear |
| Bielschowsky-PAS results | Neurofibrillary tangles and peripheral neuritis of neuritic plaques: Dark black Axons: Black Amyloid(plaque cores and vascular): Magenta Lipofuscin: Magenta |
| Seiver-Munger modification of Bielschowsky | Nerve fibers, neurofibrillary tangles and senile plaques |
| Seiver-Munger modification of Bielschowsky | Diagnoses Alzheimers |
| Seiver-Munger modification of Bielschowsky | 6-8 micrometers |
| Seiver-Munger modification of Bielschowsky control | CNS |
| Seiver-Munger modification of Bielschowsky | 1. 20% silver nitrate 2. rinse 3. Formalin 4. rinse 5, Sodium thiosulfate 6. wash 7. Dehydrate and clear |
| Seiver-Munger modification of Bielschowsky results | Nerve endings and neurofibrils: Black Neurofibrillary tangles and peripheral neuritis of neuritic plaques: Black |
| Seiver-Munger modification of Bielschowsky | Useful for demonstrating granules of some carcinoid tumor cells |
| Seiver-Munger modification of Bielschowsky | Argyrophil |
| Thioflavin S | Demonstration of neurofibrillary tangles and senile plaques |
| Thioflavin S | 10-20% NBF |
| Thioflavin S control | CNS containing senile plaques and neurofibrillary tangles |
| Thioflavin S | 1.Potassium permanganate 2. rinse 3. potassium meta bisulfite-oxalic acid 4.wash 5. sodium hydroxide-peroxide 6.wash 7. acetic acid 8.wash 9. 50% alcohol 10.Thioflavin S 11.50% alcohol 12. Dehydrate and clear |
| Thioflavin S results | Alzheimer neurofibrillary tangles, senile plaques neurites, neuropil threads, senile plaque amyloid, and cerebrovascular amyloid: Bright green Diffuse plaques and extracellular tangles: Paler yellow green PSP tangles and Pick bodies: Not well demon |
| Mallory phosphotungstic acid hematoxylin (PTAH) | Demonstration of glial cells |
| Mallory phosphotungstic acid hematoxylin (PTAH) | 10% NBF |
| Mallory phosphotungstic acid hematoxylin (PTAH) | 6-8 micrometers |
| Mallory phosphotungstic acid hematoxylin (PTAH) control | Cerebral cortex |
| Mallory phosphotungstic acid hematoxylin (PTAH) | 1. Mordant with Zenker 2. wash 3. Lugol iodine 4. decolorize 95% alcohol 5. rinse 6. Potassium permanganate 7. wash 8. Decolorize in 5% oxalic acid 9. wash 10. Stain in PTAH 11. Dehydrate and clear |
| Mallory phosphotungstic acid hematoxylin (PTAH) results | Glial fibers: Blue Nuclei: Blue Neurons: Salmon Myelin: BLue |
| Holzer method | Demonstration of glial cells in areas of gliosis |
| Holzer method | Resistant to decolorization with the alkaline aniline chloroform mix |
| Holzer method | 10% NBF |
| Holzer method | 6-8 micrometers |
| Holzer method control | Cerebral cortex |
| Holzer method | 1. Phosphomolybdic acid alcohol 2. Cover sections with absolute alcohol chloroform 3. Crystal violet 4. 10% potassium bromide 5.Blot 6. Differentiate 7. Dehydrate and clear |
| Holzer method results | Glial fibers: Blue Background: Very pale blue to colorless |
| Cajal stain | Demonstration of astrocytes |
| Cajal stain | Formalin ammonium bromide for no <2 days and no more than 25 |
| Cajal stain | 20-30 micrometers |
| Cajal stain control | cerebral cortex |
| Cajal stain results | Astrocytes with perivascular feet: Black |
| Cajal stain | 1. Gold sublimate 2. wash 3. 5% sodium thiosulfate 4. wash 5. mount, dehydrate and clear |
| Weil method | Demonstration of myelin sheath |
| Weil method | Helps in diagnosing syphilis and amyotrophic lateral sclerosis |
| Weil method | 10% NBF |
| Weil method | 10-15 micrometers |
| Weil method control | spinal cord or medullar |
| Weil method | 1. staining solution 2. wash 3. 4% ferric ammonium sulfate 4. Sodium borate potassium ferricyanide 5. Wash 6. Ammonia water 7. Wash 8. Dehydrate 9. clear |
| Weil method results | Myelin sheath: Blue to blue black Background: Light tan |
| Luxol Fast Blue | Demonstrates myelin sheath |
| Luxol Fast Blue | Is of the sulfonated copper phthalocyanine type, but is alcohol soluble |
| Luxol Fast Blue | 10% NBF |
| Luxol Fast Blue | 10-15 micrometers |
| Luxol Fast Blue control | Spinal cord or medulla |
| Luxol Fast Blue | 1. Luxol fast blue 2. rinse with 95% 3. rinse 4. Lithium carbonate 5. 70% alcohol 6. wash 7. Lithium to 70% several times 8. rinse 9. dehydrate and clear |
| Luxol Fast Blue results | Myelin: Blue to blue-green Background: Colorless |
Created by:
Ziek98
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