Save
Busy. Please wait.
Log in with Clever
or
show password
Forgot Password?
Don't have an account?  Sign up 
Sign up using Clever
or
Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account. Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't Know
Remaining cards (0)
Know
0:00
edit
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

Histology Microscopy

Topic 2: Special Microscopes

TermDefinition
Stereo Microscope 2 light microscopes combined Low magnification/Large focus More powerful than objective lens
Uses for stereo microscope Colposcope (Vaginal examination) Microsurgery Dissection (papillae) Parasitology and Pathology
Dark Field Microscope Cartoid filter: Blocks central light Object scatters light Object illuminated on a dark field
Uses for Dark Field Microscope Unstained organims Tracking movement of micro-organisms
Principle of Phase Contrast Microscope Varying thicknesses in object Varying refractive indices Microscope amplifies these variances
Theory of Phase Contrast Microscope Annulus diaphragm - Over condenser Ring of light allowed through Phase Ring - In objective lens Changes wavelength by 0.25 (lambda) Combines refracted and diffracted light
Uses of Phase Contrast Microscope Living cells Unstained cells Cell cultures
Nomarsky Microscope 2 Prisms Polarised light Prism 1: Splits light Split Light enters object close to eachother
Nomarsky Microscope (Prism 2) Prism 2 reassembles the light Contrast due to: Difference in refractive index not thickness
Uses of Nomarsky Microscope Cell cultures Unstained cells Living cells Immunostained material
Polarisation Microscope 2 filters Polarizator - Condensing lens Analizator - Objective lens
Polarizator & Analizator Filters light onto one plane Analizator is // : Field of vision is bright Analizator is perp.: Field of vision is dark
Anisotropic Structures that are able to rotate light to another plane. Shows up bright on a dark field eg. Crystals
Use of Polarisation Microscope Membrane analysis Diagnositics
Flouresence Microscope UV light source 2 filters pre and post specimen Specimen absorbs light, emits a light with bigger wavelength
Colour of self fluorescing tissues: Collagen Porifines Carotinids Lipofuscin Light Blue (Collagen) Red (Porfirines) Yellow (Carotinids/Lipofuscin)
Flourescent Dyes (F.A.R.) Flourescine Acridine Orange Rhodamine
Confocal Laser Scanning Microscope Laser beam excites molecules Laser moves across the object Light focused (w/pin hole) Records image Thicker objects
Light beams in Flouresence Microscopy Barrier 1: 450-490 nm Barrier 2: <510 nm (reflected) > 510 nm (pass) Barrier 3: 520 to 560 nm (pass through)
Created by: 10fang711
Popular Histology sets

 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!
"Know" box contains:
Time elapsed:
Retries:
restart all cards