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family bacteriaceae
Question | Answer |
---|---|
enterobacteriaceae | gram - rods/coccobacilli commensals of intestines |
enterobacteriaceae genus and species | a spectrum of biochemical types containing peaks of similar biochemical activity |
how are enterobacteriaceae species subdivided? | sub-divided by serotyping |
how are enterobacteriaceae further subdivided? | phage typing; plasmid profiles; ribosomal RNA |
habitat of enterobacteriaceae | saprophytic to parasitic |
cultural characteristics of enterobacteriaceae | facultative anaerobes |
enterobacteriaceae media requirements | NOT fastidious with many selective media |
does enterobacteriaceae have selective media? | yes, macconkey, EMB, TSI |
colonial morphology of enterobacteriaceae | all similar with variations in hemolysis, mucoid, and spreading |
T/F most lab tests were designed for enterobacteriaceae | True |
enterobacteriaceae is fermentation of lactose | LF and NLF; LF are coliforms |
antigens of enterobacteriaceae | O, H, K, F |
O antigen of enterobacteriaceae | non-spreading; somatic; from outer membrane polysaccharides; heat stable |
H antigen of enterobacteriaceae | spreading; flagellar; heat labile |
k antigen of enterobacteriaceae | capsular, carbohydrate; heat labile +/- |
F antigen of enterobacteriaceae | fimbrial; heat labile |
Major pathogens of enterobacteriaceae | escherichia coli, salmonella, yersinia, shigella |
common opportunists of enterobacteriaceae | klebsiella, enterobacter, proteus |
enterobacteriaceae virulence factors | endotoxin, capsules, fimbriae, diserophores, exotoxins, exoenzymes |
siderophores | virulence factor of enterobacteriaceae, Fe binding protein |
LF enterobacteriaceae | escherichia>klebsiella>enterobacter, citrobacter, ect |
habitat of escherichia coli | intestine |
morphology of escherichia coli | typical and motile |
cultural characteristics of escherichia coli | some beta hemalytic, Lactose fermenting |
antigens of escherichia coli | O, H, K, F |
bacterial factors of escherichia coli | fimbriae, exotoxins, hemolysins, CNF, siderophores, serum resistance, endotoxin, capsule |
e.coli F4 fimbriae | PIGLETS, age, receptors |
e.coli F8 fimbriae | calves, lambs, piglets |
e.coli F6 fimbriae | neonatal calves, pigs |
e.coli F41 | calves, lambs, piglets |
e.coli 8813 fimbriae | weaned pigs |
ecoli F107 fimbriae | "edema dz" in pigs |
e.coli vir pili adhesin | septicemia in calves, lambs |
e.coli curli adhesin | poultry, mastitis in cows |
enterotoxins of ecoli | LT, ST, verotoxin |
effects of LT enterotoxin of ecoli | diarrhea in piglets from hypersecretion |
effect of ST enterotoxin of ecoli | STa= antiabsorpitve in calves and pigs; STb stimulates secretion in pigs |
verotoxin of ecoli aka | shiga-like toxin, SLT |
effect of verotoxin of ecoli | absorbed from intestine and binds to vascular endothelium |
which enterotoxins of ecoli affect the intestines | enterotoxins and verotoxins |
pathogenesis of enterotoxins | hemolysins, cytotoxic necrotising factor, iron uptake systems, serum resistance, endotoxin, capsule |
diseases of ecoli are | intestinal or extraintestinal |
intestinal diseases of ecoli are... | enterotoxigenic or verotoxigenic |
ETEC | enterotoxigenic |
VTEC, EHEC, AEEC | verotoxigenic |
what are 2 characteristics of enterotoxigenic intestinal dz of ecoli | colonization and toxin production (LT, ST) |
what are the 2 peak times of ETEC in pigs | at <1wk age and at post weaning |
why are pigs infected with ETEC at <1wk of age | acid barrier is not yet formed, normal flora is not yet formed, receptors, sensitive cells |
why are pigs infected with ETEC at a post weaning age | villous atrophy, sows milk |
"white scours" | ETEC in calves and lambs |
at what age does ETEC occur in calves and lambs | up to 3 weeks of age YOUNG |
'edema dz' in pigs | caused by ecoli; VTEC |
what are the host factors of edema dz | over eating, genetics, stress |
VTEC in calves | hemorrhagic colitis |
VTEC in humans | hemorrhagic colitis, hemolytic uremic syndrome |
specific agent of ecoli affecting humans | O157:H7 |
how do humans become infected with ecoli 0157:H7 | animal sources-mainly beef |
EPEC | enteropathogenic ecoli |
effects of EPEC | damage to intestinal epithelium; human infants and rabbits |
what are the types of extra intestinal ecoli | extension of intestinal infection OR local contamination |
extraintestinal ecoli forms | septicemias, UTI, pyometra, mastitis, MMA, navel ill, joint ill |
what is neonatal septicemia known as | systemic colibacillosis |
most important host factor of neonatal septicemias | agammaglobulinemia |
how does neonatal septicemias occur | intestine of neonate permits passage of bacteria |
age of neonatal septicemias in calves/lambs | <1wk |
neonatal septicemias in piglets | less common, polyserositis |
epidemiology of intestinal infections | fecal-oral |
epidemiology of extraintestinal infections | various routes |
lab diagnosis of ecoli | by demonstration of the organism, heavy colonization of site |
what to keep in mind when dx ecoli | normal flora, post mortem invaders |
what do lab tests of ecoli look for | serotypes, fimbrial antigens, toxins, genes |
tx of ecoli | test the sensitivity, can be highly resistant, fluid replacement therapy |
methods for control of ecoli | hygiene, env, diet change, vaccination |
methods for prevention of ecoli | colostrum, umbilicus, quarantine, prophylaxis with care |
lactose fermenting property of klebsiella spp | lactose fermenting |
opportunistity of klebsiella | mainly opportunistic extra intestinal |
capsules of klebsiella | large |
klebsiella species | K.pneumoniae; K. pneumoniae var genitalium |
K. pneumoniae | bovine environmental mastitis, secondary cause of |
K. pneumnoniae var genitalium | causes infertility in mares; spread from stallion |
salmonella fermenting properties | non-lactose fermentating |
salmonella subgroup 1 | S. enterica subsp enterica |
host for S. enterica subsp enterica | mammals (warm blooded) |
salmonella subgroup III | S. enterica subsp arizonae |
hosts of S. enterica subsp arizonae | reptiles, cold blooded animals |
which subgroup of salmonella is zoonotic | Subgroup III; S. enterica subsp arizonae |
*naming of salmonella species | is commonly named after serotype |
what is the most common zoonosis of salmonella | salmonella enterica |
diff between ecoli and salmonella naming | ecoli after toxins; salmonella after serotypes |
habitat of S enterica subsp enterica (sbgrp I) | intestines of warm blooded animals |
habitat of S. enterica subsp arizonae (sbgrp III) | intestines of cold blooded animals OR environment |
most common test for samonella | TSI |
antigens of salmonella | O, H, and Vi |
H antigen of salmonella | phase 1 and phase 2 |
Vi capsule of salmonella | associated with virulence |
2 tests used in determining phases of H antigen | craigie tube, jamieson bridge |
pathogenesis of salmonella | host specificity, carriers, and manifestation of the disease |
bacterial factors of salmonella | enterotoxins, cytotoxins, siderophores, endotoxin, capsules, intracellular survival |
what bacterial factor makes salmonella unique | intracellular survival |
age affected by salmonella | young |
host factors of salmonella | age, normal flora, stress, carriers being active/latent/passive |
what are the carrier states of salmonella | active, latent, passive |
intestinal dz of salmonella characteristics | invasion of enterocytes, damage lamina propria, PMN, enterotoxins, diarrhea |
extra intestinal dz of salmonella | via septicemia OR local infections |
susceptibility ranking to salmonella | cattle, sheep, horses> pigs, poultry> dogs, cats |
what species does S. dublin infect | Cattle |
what are symptoms of S. dublin in cattle | enteritis, septicemia, abortion, osteomyelitis, meningitis, jt ill, terminal dry gangrene |
"dry gangrene" in cattle is caused by | S. dublin |
S. dublin is a herd problem therefore... | difficult to eradicate |
which salmonella is common in calves | S. typhimurium |
S. typhimurium is ------ in calves, ------ outbreaks, and are often caused by salepens | common-------sporadic |
which salmonellas occur in sheep and goats | S. abortus-ovis, S dublin, S montevideo, S typhimurium, S anatum |
which salmonellas occur in horses | S abortus-equi, S typhimurium |
which types of horses are particularly susceptible to salmonella spp | foals and stressed adults |
which salmonellas occur in pigs | S cholerae, S typhi-suis, S typhimurium |
which salmonellas occur in poulty | S pullorum, S gallinarum, S arizonae, S enteritidis, S typhimurium |
salmonella which causes bacillary white diarrhoea | S pullorum |
which salmonella causes fowl typhoid | S gallinarum |
zoonotic potential of salmonella | zoonotic, host adapted |
what is the zoonotic source for salmonella and why | from another animal; intracellular survival |
what is the route of infection for salmonella | fecal-oral |
what are the 2 reserviors of salmonella | animals as carriers AND egg transmission in poultry |
descibe the phages of salmonella | specific, lytic/temperate, different patterns of lysis |
what 3 lab diagnosis tech are used for salmonella | demonstration of organism, demonstration of immune response, histopathology |
what is characteristic of salmonella lab diagnosis | fewer organisms in gut and carrier state |
technique of demonstrating immune response in cattle and poultry | cattle= herd test ; poultry= target BWD for eradication |
describe histopathy in diagnosis of salmonella | suggestive but not pathogonomic |
where are live species of salmonella collected from | feces, affected tissue, blood (clotted or not) |
where are samples of salmonella collected from in the dead animal | ileum, MLN, organs |
why is antibiotic use in salmonella controversial | prolonged excretion, encourages carriers, selects resistance, prophylaxis increases susceptibility, treating horses orally makes them worse |
control and prevention of salmonella | avoid introducing inf, limit spread, vaccinate, competitive exclusion |
how is salmonella inf introduced | carrier animals, salepens, contaminated feed, commercial transport, rodents |
how do you limit spread of salmonella | early detection, quarantine, hygiene, prophylaxis |
what are the properties of the salmonella vaccines | specific, variable results, may interfere with serotesting |
nurmi principle | establishment of normal flora so salmonella may not flouurish |
what salmonella are non lactose fermenters | salmonella, proteus, shigella, yersinia |
urease reaction of proteus | positive |
growth of proteus | swarming growth on plates |
proteus lactose fermenting characteristic | NLF |
habitat of proteus | occasional extra intestinal inf especially UTI |
proteus invasiveness | rapid PM invaders |
importance of shigella in domestic animals | not important |
what species is shigella important in | humans and primates |
what was yersinia formally known as | pasteurella |
cause of bubonic plague | yersinia pestis |
what does bubonic mean | swelling of lymph nodes |
illustrator of all key concepts of bacteriology | yersinia pestis (bubonic plague) |
describe the sylvatic cycle of bubonic plague | natural cycle; is a dz of rats spread by fleas |
zoonotic potential of bubonic plague | yes |
what species has yersinia pestis | rat |
what species spreads yersinia pestis | fleas |
what is bubonic plague known as | the black death |
list the 3 stages of bubonic plague | bubonic--> septicemic--> pneumonic |
characteristics of bubonic stage | swollen burbors (LN) |
what are characteristics of septicemic stage of the plague | death |
what are characteristics of the pneumonic stage of the plague | inc # excreted |
which stages of the plague are considered rapid | septicemic and pneumonic |
characteristics of plague in cats | acute septicemia to inapparent |
zoonotic potential of plague in cats | transmission to man by aerosol, bites/scratch, fleas |
reservior of yersinia pseudotuberculosis | rodents and birds |
mode of inf of yersinia pseudotuberculosis | fecal oral |
species infected by yersinia pseudotuberculosis | rabbits, guinea pigs, rodents, birds, man, sheep |
yersinia pseudotuberculosis is commonest in ... | lab animals |
area infected and dz caused by yersinia pseudotuberculosis | mesenteric lymph nodes---acute septicemic chronic nodule |
what are carriers of yersinia enterocolitica | farm animals esp pigs |
what species does yersinia enterocolitica | chinchillas, hares, deer, simians, man |
why is yersinia enterocolita important in man | is number one cause of food poisoning |
enteric redmouth in farmed salmonids | yersinia ruckeri |