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Chapter 5
Microbiology: genetics
Term | Definition | |
---|---|---|
Genetics | The study of inheritance and inheritable traits as expressed by an organisms genetic material (rna to be used for dfferent functions) (mrna is only rna encoded) | |
Genome | The entire genetic component of an organism including its nucleotides. | |
Nucleic acid | polymers made of nucleotides (either for DNA or RNA) | |
What are nucleotides made of? | pentose sugar (deoxyribose or ribose), phosphate group, and nitrogenous base (adenine, guanine, cytosine, thymine) | |
What are the 5 nitrogenous bases? | For DNA it's adenine, guanine, cytosine, thymine. For RNA it's adenine, guanine, cytosine, uracil. | |
which of the nitrogenous bases can be complementary to each other? | A--T, G---C for DNA, and A--U for rna | |
What is located at the 2' carbon of deoxyribose? What is located at the 2' carbon of ribose | H at the 2' Ca of deoxyribose and OH of ribose | |
What structure does DNA have | DNA is a double stranded, double helical structure that is antiparallel, and is held together by hydrogen bonds between complimentary base pairs | |
What is the function of DNA? | contains the genetic material that will be used be transcribed into RNA in regions called *** | |
Genes | section that contain genetic material for an organism | |
What allows RNA to have many functions? | single stranded composed of nucleotides. Structure can vary on the type of protein and shape dictatres. | |
What are the 5 main types of RNA? What are their basic shape and function? | Coding rna (mrna) linear, tRNA carry amino acid and contain anticodon, rRNA form ribosome complex, spliceosome splice introns from pre-mrna, and regulatory RNA (help regulate gene expression | |
What rna are coding RNA? | mRNA | |
What RNA is involved in protein synthesis? | tRNA and rRNA | |
Antisense RNA (asRNA) | can bind to mRNA and prevent them from being translated | |
Splicesome RNA? | snRNA used in eukaryote pre-mRNA | |
Regulatory RNA types | Antisense RNA (blocks translation) micro RNA (miRNA) regulates translation, and small interfering RNA (silences gene expression) | |
What are the three types of RNA directly involved in the process of translation for all cells | tRNA, mRNA, and rRNA | |
What type of chromosome do bacterial cells have? single or multiple? haploid or diploid? location? plasmid present? | single circular DNA chromosome, haploid, located in the nucleoid, plasmids in the cytosol. | |
What type of chromosome do eukaryote cells have? single or multiple? haploid or diploid? location? plasmid present? | linear, multiple, diploid, located in the nucleus, plasmid found in the mitochondria | |
what are the four types of bacteria plasmids? | fertility factors, resistance factors, bacteriocin factors, virulence plasmids | |
Small Nuclear RNA (snRNA) | helps with splicing of eukaryotic mRNA during processing | |
Micro RNA (miRNA) and Small interfering RNA (siRNA) | can bind to mRNA molecules to prevent them from being translated by recruiting enzymes that hydrolyze them | |
Bacterial plasmid | small molecules of dna that replicate independently, not essential, four types of plasmids | |
eukaryotic plasmids | found in mitochondria and chloroplasts, similar to prokaryote chromosome, code for 5% of RNA and proteins, some fungi, algae, and protozoa | |
Do bacteria cells have histones? | no | |
do eukaryotes have histones | yes, located in nuclear chromosomes only | |
fertility factors | genes that are responsible for conjugation pili | |
resistance factors | genes that are responsible for antibiotic resistence factors | |
bacteriocin factors | protein secreted that are toxic to other bacteria, is competitive w/other bacteria | |
Virulence plasmids | genes that produce genes that are toxic to humans, or toxic to tissues |