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Phlebotomy study
Tools, Techniques, & Equipment
Question | Answer |
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Blood Collection Basics: (What the Standard Phlebotomy Cart/Tray contains. | Contains the following: Needles, Needle disposal containers(mini sharps box), Needle holders, Collection Tubes(various colored tops), Syringes, Butterfly needles, Marking pens, Tourniquets, Antiseptics, Gauze Pads, Bandages, gloves. |
Tourniquets: | Most commonly used is a flat strip,stretchable latex, 15-18 in. Blood pressure cuffs can also be used. |
Latex Turniquets: | Advantage is they are inexspensive and disposable. But beware of patient having allergic reactions. |
Gloves: | Latex gloves have proved to be preventive of transmission of infectious diseases. Nitrile goves have also been used. No powder. |
Needles. | The plebotomist must know two ciritical things for any given blood draw: 1) Whick kind of needle to use. 2) Which kind of tube to use. Needles should be sterile, and disposable. |
Lumen | The hollow tube within the shaft of the needle. |
Gauge | The diameter of the lumen, needle packs are color-coded by gauge for easy identification. Choice of gauge depends on the type of blood collection and the condition of the patient. |
Largest Diameter of a needle is: | 16 gauge, typically used at blood banks to collect blood. |
The smallest diameter needle used: | 23 gauge needle, used for small fragile veins. |
A 20 to 21 gauge needle: | Used when collection routine blood samples fro adult patients. |
Hub: | Place where the needle ataches to the collecting tube or syringe. |
Needle type (Multi-sample needles): | Used for most blood collections, a multisample needle is used which is a double ended needle; one tip of the needle pierces the patient' skin while the othe end is inserted into the evacuation tube. |
Needle type (Safety Syringe needles): | Patients with small or fragile veins benefit most from the use of syringe because the vacc\uum of the collection tube is likely to cause the vein to collapse; with a syringe, suction can be applied slowl and gradually, so to be more gentle. |
22 gauge | Safety Syringe needles come in a vaiety of sizes. Most commonly used is 22 gauge that is an inch long. |
Butterfly needles | Used for small and fragile veins. |
Needle Adapters | (Tube Holder) is ensured to use a good connection between them. |
A Needle Adapter | A translucent plastic cylinder with one small end that accepts the needle, while the other end has a wide opening that accepts the collection tube. Needle Adapters come in different sizes. |
Evacuated Blood Collection Tubes: | Used with both evacuated tube system and syringe method. |
Most direct and efficient method for obtaining blood specimen. | Blood collected directly into the tube. |
Evacuation Tubes | Also used for transferring blood from syringe into tubes. |
Placed in a before | Pushing a needle into the tube top in order to prevent damage to the cellular components, causes Hemolysis. |
Three components of Evacuated Tube System; | Evacuated Sample tube. Multi-Sample needle. Needle Holder. |
Double pointed needl | Enters the vein, the other end pierces the top of the tube, and the vacuum aspirates the blood. |
Color Coated Evacuation Tubes | Ensures that the physician's prescription is followed: Type of Blood Draw. Additive in the tube. Specimen to be tested. |
The Colored Tube Tops | Thick Rubber Stoppers, or with Plastic tops. Exception to plastic tubes is red-topped glass tubes. |
Red Glass Tubes | Used only for serum collection and contains NO additives; come in sizes ranging from 2 to 15 ml. |
The Evacuation Tube size is selected according to: | Age of patient. Amount of blood needed for the test. Size and condition of the patient's vein. Must be careful to match needle size to the tube size; the wrong size tube and needle combination could render the drawn sample useless. |
Some Evacuation Tubes are coated on the inside with Silicon to: | Prevent destruction of RBC's Prevent Blood from sticking to the sides of the tube. Prevent activitation of clotting factors. |
Without Additives | Blood collected in tubes without will clot and yield serum. |
Tubes that contain Additives: | May or may not clot, dependin on the type of additive they contain. |
Tubes Specifically designed to be used with | Chemistry, Hematology or microbiology instrumentation. |