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Micro - Final Exam

Stack #79343

QuestionAnswer
Mycology the study of fungi
fungus a heterotrophic thallus plant with mycelium that cannot photosyntesize; very developed cells; grows best in dark, warm, moist environments; reproduces asexually (but has ability to reproduce sexually)
heterotrophic needs organic molecules
thallus plant no stems, roots, or leaves
mycelium vegetative part; one strand = hypha (pl. hyphea)
photosynthesis using sunlight to convert water and carbon dioxide to oxygen and carbohydrates
classes phycomycota; zycomycota; ascomycota; basidiomycota; deuteromycota
phycomycota water mold; does not affect humans
basidiomycota makes mushrooms
deuteromycota not actually in class with other 4 because it hasn't been seen to reproduce sexually
phycomycota & zycomycota PRIMATIVE - aseptate hypha (fewer cell walls); spores enclosed
ascomycota & basidiomycota ADVANCED - septate hypha; spores not enclosed
how to get fungal infections (1) breath a lot of spores into repiratory tract (2) get fungi-infected dirt in open wound
spores aka - conidium (conidia)
sexual spores (1) zycomycota = zycospore (2) ascomycota = ascospore (3) basidiomycota = basidiospore
asexual spores arthrospore; chlamydospore; blastospore; sporangiospore; conidiospore; macro/microspore
arthrospore when hypha gets old, it crumbles and parts fall off; parts are called arthrospores
chlamydospore round and thick walled; resists bad conditions and germates in good conditions
blastospore (yeast) round/oval; asexual budding
sporangiospore [zycomycota and deuteromycota] continue to make spores enclosed in sprangium until it bursts and spores and spread; includes: sporangiospore, sporangium, sporangiophore, and primative hypha
conidiospore [asomycota and deuteromycota] includes: conidiospore, conidium, conidiophore, and advanced hypha
macro/microspore causes athlete's foot; infects hair, skin, and nails; in group called dermatophytes
diseases caused by fungi athlete's foot and yeast infection
athlete's foot to treat: dry up moisure and go barefoot; create uninviting enviornment to prevent growth
yeast infection only in females; caused by Candida albicans; normal vag flora in small amounts - overgrowth = disease; antimicrobial agent can cause imbalance b/c it kills bacteria and allows yeast to grow
Virology the study of viruses
virus very tiny intracellular parasite; can be seen by light microscope - must use electron microscope; has to live inside a living cell
virus vs bacteria much smaller; need host cell; no organells or ribosomes; no metabolism; either DNA or RNA (not both); either single or double stranded; no motility
shapes icosahedron; helical; complex
icosahedron has 30 edges, 20 faces, and 12 apexes; outer edge is capsid made of protein and capsomes make up capsid; if no envelope = naked - attaches to host by capsid
helical includes: casomer, capsid, and nucleic acid (often infects plants)
complex attacks bacteria; includes: head, collar, tail, tail fiber, pins, base plate; tail fiber holds bacteria, pins puncture cell wall, tail compresses and nucleic acid is forced through hole in cell membrane into bacteria
infecting a host cell lytic cycle and lysogenic cycle
attachment attach to host cell
penetration virus gets inside cell - usually host cell pulls virus in entirely (not complex): called pinocytosis
uncoating (lytic) capsid or envelope releases nucleic acid
uncoating (lysogenic) viral nucleic acid joins with bacterial nucleic acid so when host cell replicates, viral nucleic acid is part of new cell; dormant until triggered to complete cycle
multiplication/biosynthesis different for each virus; many steps combined - takes over host cell and used host's ATP and ribosomes to make new virus parts, then parts randonly assemble
burst when virus comes out of host cell
burst: EXPLOSIVE virus kills host cell
burst: BUDDING virus leaks out and host cell is not killed
burst size how many new virusesare made from one virus in one host cell
burst time how long it takes from attachment to burst
How to destory a virus autoclave (high heat, pressure, and steam); disinfect; chlorine bleach; heat; acetone or alcohol (good against enveloped cell); UV light (good against single stranded virus - mutated); antimicrobial agent has NO effect
How to grow a virus in virology lab - 37C constant temperature; use human cells in bottle and pass from bottle to bottle until death
cancer cell do not die - can last 1000s of passages; no contact inhibitory - layer of cells
normal cell die after about 50 passages; monolayer - contact inhibitory don't grow on top of each other
cytopathic effect (CPE) change a virus makes on its host cell
disease transmission respiratory route; oral/fecal route; direct contact; injection
Created by: lcnelson
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