Ag enviro 2 lecture Matching
| DNA polymerase (Taq) is |
| stable at 95 degrees |
| step 3 of sequencing invovles |
| adding DNA polymerase |
| polyacrylamide gels are used to separate |
| segments differing by very few nucleotides |
| to make a transgene animal either |
| inject it into the proembryo nuclei or do a nuclear transfer |
| step 4 of sequencing involves |
| Adding dNTPs such as dATP, dTTP, dGTP, dCTP |
| DNa is read from the |
| 5' end to the 3' end |
| DNA moves from |
| negative to positive poles during electrophoresis |
| polyacrylamide gels are used to |
| separated molecules which might differ by as little as one base |
| a plasmid |
| a loop of DNA found in bacteria |
| DNA is cut using |
| restriction enzymes |
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