Protein Analysis
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| Give two reasons why protein analysis is important in nutrition labeling. | show 🗑
|
||||
| show | Nitrogen content, peptide bonds, aromatic amino acids, dye binding capacity, ultraviolet absorptivity
🗑
|
||||
| show | Kjeldahl
🗑
|
||||
| T or F: The Kjeldahl methods measures inorganic nitrogen. | show 🗑
|
||||
| ____ methods utilizes combustion and gas chromatography to measure nitrogen. | show 🗑
|
||||
| show | True
🗑
|
||||
| show | IR
🗑
|
||||
| In the BCA method, proteins reduce _____ ions to _____ ions under alkaline conditions and these ions react with BCA to give a purple color. | show 🗑
|
||||
| What four amino acids does BCA depend on? | show 🗑
|
||||
| What three amino acids absorb UV light at 280 nm? | show 🗑
|
||||
| show | True
🗑
|
||||
| show | True
🗑
|
||||
| show | Extraction under alkaline conditions and then precipitation.
🗑
|
||||
| T or F: Nonprotein nitrogen can cause overestimation of protein efficiency ratio. | show 🗑
|
||||
| show | Biochemical characteristics, nutritional quality, functional properties
🗑
|
||||
| show | Solubility, thermal stability, adsorption characteristics, charge, size
🗑
|
||||
| Describe salting out. | show 🗑
|
||||
| show | Proteins precipitate at their isoelectric point because there is no electrostatic repulsion.
🗑
|
||||
| Describe solvent fractionation. | show 🗑
|
||||
| show | Heat or extremes in pH cause precipitation.
🗑
|
||||
| What are the four methods of differential solubility? | show 🗑
|
||||
| In ____ chromatography, adsorption occurs between charged solid molecules in solution and the charged matrix. | show 🗑
|
||||
| In ____ chromatography, ligands bind to the support so that proteins can bind to the ligands; changing the pH allows for elution. | show 🗑
|
||||
| show | Dialysis
🗑
|
||||
| Name three types of membrane processes which separate based on size under pressure. | show 🗑
|
||||
| show | True
🗑
|
||||
| show | SDS-PAGE, isoelectric focusing, 2D electrophoresis
🗑
|
||||
| Describe SDS-PAGE. | show 🗑
|
||||
| show | SDS-PAGE
🗑
|
||||
| Describe isoelectric focusing. | show 🗑
|
||||
| Describe 2D electrophoresis. | show 🗑
|
||||
| show | Amino acid analysis
🗑
|
||||
| Describe amino acid analysis. | show 🗑
|
||||
| T or F: Chromatography does not require the use of an internal standard or standard curves. | show 🗑
|
||||
| show | Amino acid composition, digestability, anti-nutritional factors (eg. enzyme inhibitors/tannins)
🗑
|
||||
| How is protein efficiency ratio determined? | show 🗑
|
||||
| show | Essential amino acid index, in vitro protein digestability, lysine availability
🗑
|
||||
| show | Solubility, emulsification, foaming, gelation, dough formation
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
goberoi
Popular Agriculture sets