Core Practicals for Biology
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| show | 1.Place Daphnia on cavity slide with caffeine
2.Idea of heart rate determined before
treatment ;
3.Idea that daphnia need to be put into Caffeine and allowed to acclimatise ;
4.Use microscope; to check heartbeat
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| show | 5.Range of caffeine concentrations;
6.Repeats;
7.Water as control / zero caffeine concentration};
8.Control temperature;
9.Similar organisms used;
10.Accurate measurement of DV;
11.Plot results on graph
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| Describe how to investigate the vitamin content of food and drink | show 🗑
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| Describe how to investigate the vitamin content of food and drink [2] | show 🗑
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| show | 1.Tissue named e.g. beetroot ;
2.Washing/soaking beetroot thoroughly
3.Waterbath to maintain change temperature
4.Range at least 5{temperatures / alcohol concentrations}
5.Controlled variable named e.g. length of time, size of beetroot
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| show | 6.Indication of what is being used to judge permeability colour of solution, absorbance, transmission ;
7.Permeability can be assessed e.g. use of colorimeter, standard solutions ;
8.Repeats / replicates
9.Plot results onto a graph
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| show | Mesure of vol. of oxygen. volume of oxygen
3.Measure the initial rate of reaction
4.CV e.g. age, part of mussel mass,surface area,Vol of hydrogen peroxide, temperature, concentration, pH
6.Repeats
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| Describe the stages of mitosis and how to prepare and stain a root tip squash in order to observe them practically | show 🗑
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| show | 1.Sample/explants from both(tissues)
2.Aseptic cond.
3.Grow cells in a callus
4.Growth reg.
5.Cells / tissue can differentiate / cells can become whole plants
6.Procedure e.g. agar; leave for a suitable length of time / suitable controlled variable
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| show | 1.Suspending fibres with weights,pulling them with forcemeter
2.Fibres of same diameter,different diameters accounted
3.Fibres of initial length
4.How weights added/forcemeter pulled
5.Measurable endpoint, breaking point stretched to standard length
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| show | 5.Measurable endpoint eg breaking point /
stretched to standard length
6.Repeated readings taken at each {weight / forcemeter reading}
7.Safety procedure eg goggles;fibre
snaps/precaution against weights};
8.Control of {temperature / humidity
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| show | 1.3+ range of ion concentrations
2.Repeats(at each concentration)
3. Uniformity of seedlings (e.g. all
from same parent plant, same age, same
original root length)
4.Solution used should contain other
mineral ions / named mineral ions
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| Describe how to investigate plant mineral deficiencies practically. [2] | show 🗑
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| Describe how to investigate the antimicrobial properties of plants. | show 🗑
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