Detection of MOs Word Scramble
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| Question | Answer |
| What are two books referenced for microbiological methods? | FDA BAM, USDA Microbiology Guidebook |
| What is the purpose of using Petrifilms? | A ready-to-use alternative to traditional enumeration media; does not require as much prep. |
| What is VIDAS? | An automated immunoassay system. |
| VIDAS uses ELFA, which stands for what? | Enzyme Linked Fluorescent Assay |
| VIDAS allows for specific detection of a ____ or _____. | toxin, antigen |
| T or F: If a VIDAS result comes back positive, it means the product definitely is contaminated. | False |
| T or F: VIDAS results need to be confirmed culturally or biochemically. | True |
| Describe the MPN technique. | Multiple dilutions of a sample are made and assessed for turbidity. The number of positive tubes corresponds to a MPN/100 mL. |
| MPN is based on the ______ distribution. | Poisson |
| Define MPN. | A statistical estimate of concentration. |
| What is TEMPO? | An automated quality indicator system which does MPN for you. |
| What is the final result of TEMPO expressed in? | CFU/g |
| Name three methods of conventional identification. | Selective/differential media, serological testing, biochemical tests |
| Name three methods of rapid testing. | PCR, ATLAS, Vitek |
| What are the three components of PCR? | DNA extraction, DNA amplification, DNA detection |
| What are the steps involved in PCR? | Denaturation, annealing, extension |
| qPCR uses ______ in order to detect levels of _____ _____. | fluorophores, gene expression |
| What three biochemical methods are used for bacteria identification? | Growth media, serological tests, protein analysis |
| What is Vitek used for? | Identification of bacteria and antibiotic susceptibility. |
| T or F: MPN is particularly useful for high concentrations of organisms. | False |
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goberoi
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