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Virology Basics
Animal Virology Basics
| Question | Answer |
|---|---|
| Capsid def | the protein shell that surrounds the nucleic acid genome |
| 3 roles of capsids | 1:Protect genome, 2: Packages genom, 3:Facilitate interaction with host. |
| 2 roles of nucleic acid: | !: Viral genetic info, 2: Can encode for virus-specific macromolecules. |
| nucleocapsid def | when virus has 2 capsids, it is the inner one, closest to the NA |
| Capsomere def | Each repeating unit of the capsid |
| 5 Families of DNA viruses (w/ exemples) | Parvo (B19), Adeno (adenovirus), Herpes (HSV, VZV), Pox (smallpox), Papova (papilloma) |
| 6 Families of ssRNA+ viruses (w/ exemples) | Picorna (polio), Flavi (yellow fever, west nile), Toga (Rubella), Astro (astrovirus), Calici (norwalk), Corona (coronavirus) |
| 4 Families of ssRNA- viruses (w/ exemples) | Paramyxo (measles, RSV) Rhabdo (rabies), Filo (ebola), Borna (bornavirus) |
| 1 family of ssRNA- SEGMENTED (w/ exemple) | orthomyxo (influenza type A) |
| 2 families of ssRNA AMBISENSE SEGMENTED (w/ exemples) | Bunya (hanta), Arena (lassa fever) |
| 1 family of dsRNA SEGMENTED (w/ exemple) | Reo (rota) |
| 2 families of Reverse transcriptase viruses (w/ exemples) | Retroviridae (HIV), Hepadnaviridae (HBV) |
| pros/cons of primary cell culture | Pro:Closest to tissue of origin Con: very few generations, expensive, not same cell type since have to get new cells |
| pros/cons of diploid cell culture | pro: comes from a single cell, clonal, 50 generations, diploid Con: have to get new cells after 50 gen, not same as primary cell (undergone mutation), |
| pros/cons of continuous cell culture | Pro: immortal, cheap Con: aneuploid, unknown background |
| Difference between biological and physical method of virus detection | bio: how much live or infectious virus is present in sample (better method but slower and expensive). Physical: how much total virus is present. |
| if there are X plaque forming units in 0.1mL of a 10^4 dilution whats the titer of the virus stock? | 10X in 1mL of 10^4 so X x(10^5) |
| 2 Examples of biological methods of detection | Cytopathic effects (rounding, fusion, inclusion bodies, lysis), Plaque Assays |
| 4 Exemples of physical methods of detection | particle counts with electron microscopes,hemagglutination assays, Elisa (and RIA) and application of Virus-specific antibody, southern blots, PCR |
| What is the eclipse period? | The period of time between infection by a virus and the appearance of the mature virus within the cell. |
| What is the latent period? | The interval between exposure to an infectious organism or a carcinogen and the clinical appearance of disease |
Created by:
harrysomerset
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