Save
Busy. Please wait.
Log in with Clever
or
show password
Forgot Password?
Don't have an account?  Sign up 
Sign up using Clever
or
Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account. Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't Know
Remaining cards (0)
Know
0:00
edit
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

Micro-Lab002

Aerotolerance

QuestionAnswer
What is thioglycolate broth used for? To differentiate oxygen requirements.
Where do obligate aerobic (oxygen-needing) bacteria gather on a thioglycolate broth? Why? at the top of the test tube in order to absorb maximal amount of oxygen.
Where do obligate anaerobic bacteria gather on a thioglycolate broth? Why? at the bottom, to avoid oxygen.
Where do facultative bacteria gather on a thioglycolate broth? Why? at the top, since aerobic respiration is the most beneficial one; but, as lack of oxygen does not hurt them, they can be found all along the test tube.
Where do microaerophiles gather on a thioglycolate broth? Why? at the upper part of the test tube but not at the top. They require oxygen but at a low concentration
Where do aerotolerant bacteria gather on a thioglycolate broth? Why? they are evenly spread along the test tube because they aren't affected at all by oxygen.
Why don't you shake the thioglycolate broth? Shaking would add more oxygen to the tube than thioglycolate could absorb
What should you do to salvage it if you do shake it? Vigorous heating will boil out dissolved gases
What causes condensation on the sides of the anaerobic jar? Basically: H20-2 hydrogen's, 1 oxygen. Water is formed from 2 hydrogens attaching to every oxygen molecule to form water. Answer on paper: atmospheric oxygen and hydrogen liberated from the GasPak.
Why can't you do a catalase test on organisms grown on blood agars? Blood cells are catalase positive and will bubble if H2O2 (hydrogen peroxide) is added, regardless of whether or not the organism growing on top of it is catalase negative or not.
What is the oxygen requirement category for: Alcaligenes aerobe
What is the oxygen requirement category for: Clostridium obligate anaerobe
What is the oxygen requirement category for: Enterococcus aerotolerant anaerobe
What is the oxygen requirement category for: Escherichia facultative anaerobe
Can aerobic bacteria grow in the absence of Oxygen (O2)? Why or why not? Yes, because aerobic bacteria may be able to use fermentation or anaerobic respiration.
What would you need to do to determine whether bacteria growing on a Petri plate (agar)from the Brewer jar (Jar with tight lid that stunk really bad when opened in class) are anaerobes (don't like oxygen)? Subcultures from these colonies could be incubated aerobically (with oxygen) to determine whether they can grow in the presence of oxygen. If they can't, they're most likely anaerobic.
What does the appearance of a blue or pink color in a thioglycolate tube mean? The blue or pink area on the surface is the area that's been exposed to the oxygen. It means the indicator has been oxidized (combined with oxygen)
Why will obligate anaerobes grow in thioglycolate? The free sulfur will tie up atmospheric oxygen, leaving the bottom of the medium anaerobic.
To what do you attribute the odors (stench) of anaerobic (no air) decomposition? To the organic molecules produced that have odors. These molecules include: indoles acids alcohols amines ketones inorganic compounds (ammonia & hydrogen sulfide)
Why is aerobic (with air) decomposition not stinky? Produces only inorganic molecules, which are odorless.
How do oxygen requirements differ between Streptococcus and Escherichia, which are both described as facultative anaerobes in Bergey's Manual? Streptococcus: stricly fermentative. Lacks an electron transport chain. Escherichia: can grow anaerobically by fermentation and aerobically by using oxygen as the final electron acceptor in respiration.
Between Streptococcus and Escherichia, which one could correctly be called an aerotolerant anaerobe? Streptococcus
How can the aerobe Pseudomonas aeruginosa grow in the absence of oxygen? Some strains of Pseudomonas aeruginosa are capable of using NO3– as the final electron acceptor in anaerobic respiration
The catalase test is often used clinically to distinguish two genera of gram-positive cocci,(Blank A) and (Blank B)It is also used to distinguish two genera of gram-positive rods (Blank c) and (Blank D) Blank A: Streptococcus (catalase-negative) Blank B: Staphylococcus (catalase-positive) Blank C: Bacillus (catalase-positive) Blank D: Clostridium (catalase-negative)
Gram-positive rods with endospores example? Clostridium
Gram-positive rods with no endospores example? Lactobacillus
Gram-positive cocci example? Enterococcus
Gram-negative rods with pointed ends example? Fusobacterium
Gram-negative rods with blunt ends example? Bacteroides
Gram-negative cocci example? Veillonella
Created by: 100001455498513
Popular Science sets

 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!
"Know" box contains:
Time elapsed:
Retries:
restart all cards