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BNS 305 1
Methods and usages, voltage and current, nernst equation practice problems
| Question | Answer |
|---|---|
| What is the central dogma of molecular biology? | DNA --translation---> mRNA--transcription---> PROTEIN |
| What does PCR do? When would you use it? what are some pros/cons? | PCR-polymerase chain reaction: look for the presents of genetic material at the DNA level (looking at numbers of base pairs) -replication of specific part of genome -cheap and easy (+) -Needs analysis after procedure (gels) |
| What is sequencing? What are some different types? what is the shotgun approach? What are some advantages/disadvantages/ | Sequencing is looking at the base pairs (ATCG) that make up a fragment of DNA. --> need PCR later on. These methods can be expensive. Next Gen/Shotgun: whole genome sequencing using small pieces and replicating them -Sanger: small-cheap |
| what is ChIP? what is it used for? what is are some pros/cons? | ChIP- CHROMATIN immunoprecipitation. It is used to asses chromatin and study the epigenetics of an organism (the way the genetic code changes) output is DNA -->NEED PCR OR BLOT AFTER uses ANTIBODIES for proteins that capture DNA they're associated with |
| what is ATAC-Sequencing? Why is amplification of transcribed sections different for different cells? | Assesses chromatin- uses enzyme (Tn5) to mark open chromatin and marked bits are amplified. ONLY actively transcribed bits are replicated -- different cells have different functions and create diff proteins for them |
| what does qPCR do? when would you use it? what are some pros/cons? | RNA |
| what is In situ-Hybridization? what does it tell you? pros/cons? | RNA |
| How do you study TRANSCRIPTION? pros/cons? | RNA sequencing -measure RNA in cell |
| What is Drop-seq? what does it tell you? | RNA |
| What does Immunohistochemistry tell you? How does it work? pros/cons? When would you use this? | PROTEINS- antibodies |
| What is Fluorescence (technique)? How does it work? When would you use this? | MOLECULES/PROTEINS |
| What does a Western Blot tell you? pros/cons | PROTEIN amounts-antibody dependent quality |
| What is Proteomics? what does it tell you? | All Proteins |
Created by:
KylaG
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