click below
click below
Normal Size Small Size show me how
tubes & tests
order of draw, common tests
Question | Answer |
---|---|
name the order of draw for multiple evac tube collection | blood culture, coagulation (blue), serum (red, gold SST), heparin (green), EDTA (lavender), glycolytic inhibitor (grey) |
when are sterile blood samples taken | always, first regardless of the method |
name the order of draw for multiple syringe collection | blood culture, coagulation (blue) EDTA (lavendar), Gel separator (SST, PST) or non additive (red) |
what colour tube does Hematology mainly use? | EDTA - lavendar |
what colour tube does chemistry mainly use? | light green - PST (gold SST is acceptable) |
what colour tube does coagulation studies mainly use? | Light Blue - sodium citrate |
Gold tube has | gel separator and clot activator. referred to as SST. |
Light Blue tube has | sodium citrate anticoagulant |
Red tube has | clot activator and NO anticoagulant |
Dark Green tube has | sodium heparin anticoagulant |
Light Green tube has | lithium heparin anticoagulant and Gel separator, referred to as PST. |
tubes containing anticoagulants: | dark green, light green, light blue, black, royal blue, lavender |
tubes containing gel separator: | gold-SST, light green-PST |
tubes containing clot activator & no anticoagulant: | red (plastic) |
colour of tube to be used for CBC | lavender |
colour of tube to be used for lead | royal blue (w lavender band) |
colour of tube for PT, PTT | light blue |
colour of tube to be used for K, CREA, GLUF, GLUR, ALP, ALT, CA, CK, LIP, TP, UREA | light green (mint) -PST |
colour of tube to be used for Acute Hepatitis screen | gold - SST |
colour of tube to be used for SPE (serum protein electrophonesis) | gold - SST |
colour of tube to be used for RUBG, TTG, RF, LDL, HDL | light green (mint) - PST |
colour of tube that must be filled correctly/ in proper ratio of 9:1 | light blue |
most common hematology tests and colour of tube used: | CBC - includes: RBC, WBC< platelet count, Hgb, Hct & diff leukocyte count. Lavender tube with EDTA |
RBC test? | determines the number of circulating red blood cells |
WBC test? | determines the number of leukocytes |
platelet count? | determines the number of platelets in the peripheral blood. Used to diagnose bleeding disorders and monitor anticoagulant therapy. |
Hgb (hemoglobin) test? | a low value can indicate anemia, high value may occur with sever burns. It is the O2 carrying protein found in rbc's |
Hct (hematocrit) test? | the ratio of rbc's to whole blood volume. Low value may indicate anemia or hemorrhage, elevated value may indicate dehydration or polycythemia. Also called packed cell volume. |
Differential leukocyte count? | count of each wbc, neutrophil, eosinophil, basophil, lymphocyte and monocyte. |
ESR (erythrocyte sedimentation rate)? | measure the rate at which erythrocytes fall to the bottom of a tube under controlled lab conditions. Use the Westergren method. |
hemostatsis? | the process by which bleeding is stopped |
hematopoesis? | process by which erythrocytes are formed and developed in bone marrow |
plasma? | a straw coloured liquid includes : fibrinogen, proteins, Na, K, Cl etc. electrolytes, globulin. |
serum? | same components as plasma except fibrinogen is removed by allowing clotting to happen then centrifuged to separate cells from serum. |
erythrocyte? | largest cell, red in colour |
leukocyte? | second largest cell. there are two types - granulocytes & agranulocytes. |
granulocytes? | they have granules, 3 types: neutrophils, basophils & eosinophils. |
which two granulocytes play a role in allergic reactions? | basophil and eosinophil |
agranulocytes? | hav no granules, 2 types: lymphocytes and monocytes |
Phagocytic cells that engulf bacteria and dead tissue cells? | neutrophils & monocytes |
the cell that produces antibodies against substances the body recognizes as foreign? | lymphocytes |
thrombocyte? | also known as a platelet, not a true cell but a cell fragment. Plays a major role in the clotting process by forming the platelet plug that seals breaks in blood vessels. |
Four main tests in the Coagulation section of Hematology department? | platelet count, bleeding time test, PT (prothrombin time test or protime) & APTT (activated partial thromboplastin time) |
what is a timed specimen? | some tests need to be done at a specific time or after a set of criteria is met. Ex. STAT, pre-op, fasting. |
criteria for fasting specimens? | patient eats or drinks nothing for a period of time (8-12 hours). Draw. Common tests: cholesterol, triglycerides & glucose determinations. |
Two hour postprandial glucose test (GLUC2H) | Blood drawn 2 hours after eating. Light green tube. Screening test for diabetes. |
Glucose tolerance test? | Blood drawn on fasting patient, after drinking a standard amount of glucose. Samples taken at periodic intervals. Urine is also collected. Test for: diabetes mellitus & hypoglycemia. |
Glucagon tolerance test? | tests liver for stored glycogen. Fasting 12 hours, injected with glucagon, another spec is drawn. |
Therapeutic drug monitoring (TDM)? | measures the amount of a particular drug in a patient's bloodstream. Good for optimal dosing amounts. Performed on serum drawn in a plain red tube, kept upright, time and method of draw must be included. |