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Forensic 2 Sem1
Review for Forensic Science 2 from 8/12/24 - 12/20/24
Question | Answer |
---|---|
What are some common hazards in forensic science? | Infectious human blood and bodily fluids, toxic chemicals, hypodermic needles, broken glass, other sharps |
What are the routes of exposure to hazards in forensic labs? | Inhalation, skin contact, ingestion, injection |
What are the federal and state regulations related to laboratory safety? | Personnel must be informed and trained on chemical hazards, supervisors ensure safety precautions are followed |
Who develops, implements, and enforces safety standards in forensic science? | Occupational Safety and Health Act of 1970, National Institute for Occupational Health (NIOSH), Environmental Protection Agency (EPA) |
What are chemical hazards? | Corrosive materials, radioactive substances, carcinogens, biohazards, compressed gases, toxic chemicals, reactivity chemicals, flammable substances |
What health and safety hazards are present in forensic labs? | Flammable materials, explosive materials, pyrophoric materials, oxidizers, corrosive materials |
What information is found in a Material Safety Data Sheet (MSDS)? | Hazardous properties, disposal techniques, personal protection, packaging and shipping procedures, emergency procedures for spills/exposure |
How are chemicals labeled for safety? | Labels are the primary warning source, with mandated information from federal and state regulations |
How are chemical hazards portrayed on labels? | Picture hazard, symbol hazard, word hazard |
How is chemical waste collected and disposed of? | EPA regulates disposal; improper disposal can lead to fines or jail |
What are biological hazards in forensic science? | Body fluids, tissue, and biological specimens that may contain bloodborne pathogens like HIV, AIDS, and Hepatitis |
What are universal precautions in forensic labs? | Treat all body fluids and materials as if infected, use engineering controls like biohazard bags, puncture-resistant containers, long-handled mirrors |
What protective measures should be taken in forensic labs? | Barrier protection (gloves, coveralls, shoe covers), eye/face protection, sharp containers, no eating/drinking/applying cosmetics, hand washing, respirators for airborne hazards |
How can contamination and transfer be avoided in forensic labs? | Decontaminate tools/equipment with disinfectants (e.g., bleach diluted 1:10, 70% isopropyl alcohol), allow sufficient contact time |
How is biological waste handled and disposed of? | Biohazardous waste (cadaver waste, human blood/tissues) must be stored and disposed of separately from regular waste |
What are other classes of hazardous materials in forensic labs? | Carcinogens (cancer-causing agents), teratogens and mutagens (cause DNA mutations/genetic damage), compressed gases (flammable/explosive), radioactive materials (used in assays), cryogenic materials (e.g., liquid nitrogen) |
What are the types of Personal Protective Equipment (PPE)? | Hand Protection, Eye Protection, Foot Protection, Respiratory Protection, Head Protection |
What is the purpose of hand protection in PPE? | Selected based on material hazards, checked for tears or holes, minimize contamination while wearing gloves |
What is required for eye protection in the lab? | Safety glasses/goggles for biological, chemical, and radioactive materials, face shields for splashing or flying debris, no contact lenses, worn over prescription glasses |
What is required for foot protection in the lab? | Shoes covering and protecting the feet, used to prevent injuries from falling objects, electrical hazards, and contamination, non-permeable shoe covers can be used |
When is respiratory protection necessary in the lab? | In bombings, clandestine laboratories, and when organic solvents produce noxious fumes or airborne contaminants |
What is head protection used for in the lab? | Required at crime scenes like bombings where structural damage may have occurred |
What laboratory safety equipment is required by federal laws? | Safety shower, fire-extinguishing materials, blankets, handheld extinguishers, hoses, eye bath, first aid kits |
What is the function of a ventilated fume hood? | Extracts fumes and hazards away from technicians and vents them to a safe area |
What are the laboratory exposure control plans for chemical spills? | Procedures for handling acid, mercury, solvent, radioactive chemical spills, warn personnel and minimize contamination spread |
What is a chain of custody (COC)? | An unbroken trail of accountability ensuring the security of samples, data, and records, including signatures or initials, dates, and identifiers for each transfer |
Why is a chain of custody necessary? | It reduces sample mix-ups, contamination, tampering, and ensures legal defense by showing accurate analytical procedures and verifying sample identity |
What should be included in a chain of custody during transportation? | Name and address of requesting company, list of samples with IDs, type of sample, manner of collection, tests requested, name of testing lab, relinquishing and receiving signatures |
What must be done when receiving samples at the lab? | Verify sample integrity, ensure documentation is complete, confirm sample IDs match, check preservative/storage temperature, and refuse samples if there are discrepancies |
What information should be logged for evidence? | Date collected, date received, unique lab ID number, original field number, required analyses, and signature/initials of person logging |
How should samples be stored to ensure proper handling? | Store samples to minimize degradation/contamination, secure from tampering, and in an area with limited access |
Should the sample log be computerized or handwritten? | This depends on lab policies; both methods can be used, but maintaining secure, accurate logs is essential |
What does questioned document examination involve? | Examining writing materials, inks, alterations, sequencing of writing, paper and ink comparison, and identifying authorship or source of the document |
What are some crimes associated with questioned documents? | Contested wills, forgery, embezzlement, breaches of contract, extortion, robbery, suicide, homicide, kidnapping |
How should document evidence be collected? | Avoid excessive handling, place in a clean envelope, label appropriately, and mark containers, not documents, unless absolutely necessary |
What are exemplars in questioned document examination? | Known samples of handwriting or materials used for comparison with questioned material |
How should exemplars be chosen? | Use similar writing instruments, paper, and wording; collect with minimal distraction and ensure natural writing variations |
What are the types of writing instruments identified in questioned documents? | Ballpoint pens, fountain pens, fiber-tip pens, pencils |
What characteristics are examined for paper identification? | Color, size, shape, watermarks, thickness, weight, inclusions, surface appearance, and fluorescence |
What are the common ink classifications? | Ballpoint, liquid, printing, and typewriter inks |
How is ink analyzed and compared? | Through color comparison, chemical composition, infrared absorption, luminescence, and thin-layer chromatography |
How is ink dating done? | Using high-performance liquid chromatography or gas chromatography-mass spectroscopy to measure volatile components in ink |
What does handwriting comparison involve? | Analyzing individual and class characteristics to determine if questioned writing matches known samples |
What are class characteristics in handwriting? | Gross features like letter forms, letter dimensions, punctuation, and connections between letters |
What are individualized characteristics in handwriting? | Unique features like characteristic letter forms, strokes, and flourishes developed over time |
What factors affect handwriting characteristics? | Speed of writing, health, age, drug or alcohol use, and attempts to disguise writing |
What additional factors should be considered in handwriting? | Spacing, alignment, margins, spelling, phraseology, and grammar |
How are photocopies analyzed in questioned document examination? | Determining the type of photocopying process, the machine used, and whether specific copies were made by the same machine |
What are the methods of photocopying processes? | Chemical (light-sensitive paper), thermal (heat), and electrostatic (selenium-coated metal plate) |
How are alterations, erasures, and obliterations examined? | Using fingerprint powder, UV or infrared light, and comparing writing materials or spacing |
How is indented writing analyzed? | Using oblique lighting, fluorescent powder, electrostatic document apparatus (ESDA), or iodine vapor |
What are the methods for counterfeit currency detection? | Examining the weight of paper, watermarks, metal strips, colors, and intricate designs |
What is digital evidence? | Digital evidence includes cameras, computers, laptops, tablets, cell phones, flash drives, MP3 players, calculators, and digital planners |
Why is examiner training important in digital evidence examination? | Examiners must be trained to follow accepted forensic procedures and conduct proper examinations |
What are the four steps in the digital evidence examination process? | Preparation, Extraction, Analysis, and Conclusion |
What is involved in the preparation of digital evidence? | Prepare a working directory on separate media for evidentiary file recovery or extraction |
What is the difference between physical and logical extraction? | Physical extraction recovers data across the entire drive, ignoring file systems. Logical extraction recovers data based on the file system present on the drive |
What methods are used in physical extraction? | Keyword searching, file carving, and extraction of partition tables and unused space |
What is keyword searching in digital evidence examination? | A method used to search for specific keywords across the physical drive to identify data not accounted for by the operating system |
What is file carving? | A technique that recovers usable files and data from a drive, even if the files are not listed by the operating system |
What is logical extraction used for? | Extracting data from areas like active files, deleted files, file slack, and unallocated space, based on the file system present |
What types of data are extracted during logical extraction? | Data from the file system such as directory structure, file attributes, file names, timestamps, file sizes, and locations |
What is timeframe analysis? | Analyzing timestamps and system logs to determine when specific events occurred on the system |
What is data hiding analysis? | Detecting concealed data, such as mismatched file headers/extensions, encrypted files, or hidden partitions |
What is steganography in the context of digital evidence? | The practice of hiding data within files or systems, which can be detected during data hiding analysis |
How can ownership and possession be determined in digital evidence? | By analyzing timestamps, file names, hidden data, and passwords to link files to a specific user |
Why is drawing conclusions important in digital evidence examination? | Individual findings may not be sufficient on their own; conclusions must be based on the totality of the extracted data and analysis |
What term describes all physical materials examined in the forensic laboratory? | Physical evidence (synonym for trace evidence) |
What are common collection methods for trace evidence? | Lifting (using tape), scraping (dislodging materials onto clean paper), vacuum sweeping (vacuum with a filter trap), combing (comb or brush hair and debris), clipping (fingernails and scrapings packaged separately) |
Why is microscopy used in forensic analysis? | To view and compare small evidence |
What are types of microscopes used in forensic analysis? | Basic low power microscope, specialized microscopes, comparison microscope |
What is a comparison microscope? | An instrument that uses two compound microscopes joined by a bridge to view images side-by-side |
What are some specialized microscopes used for trace evidence analysis? | Low power stereo microscope, polarized light microscope, phase contrast microscopes, scanning electron microscopy, X-ray fluorescence spectrometry, X-ray diffraction, vibrational spectroscopy, pyrolysis gas chromatography, microspectrophotometry |
What are some factors considered when analyzing forensic paint? | Case issues, sample size, complexity and condition, environmental effects, sample collection methods |
How is paint typically analyzed in forensic cases? | Sample prep, layer analysis, UV-visible microscopy, infrared spectrophotometry, electron microscopy |
How is glass identified in forensic analysis? | A: By three physical characteristics: conchoidal fracture, amorphous structure, isotropism |
What are the layers of a human hair? | Medulla (inner layer), Cortex (middle layer), andCuticle (outer layer) |
What is the typical hair growth cycle? | Anagen: Growth phase, Catagen: Shrinking phase, and Telogen: Resting phase |
How is hair examined in forensic investigations? | By determining color, length, diameter, pigment granules in the cortex, medulla structure |
How can the origin of hair be determined? | By examining the cuticle, cortex, and medulla, and considering species and racial origin |
What are the two types of fibers commonly encountered in forensic analysis? | Natural fibers (from plant or animal materials, e.g., cotton, wool, silk), synthetic fibers (man-made, e.g., nylon, polyester, rayon) |
What is fingerprint examination? | The study, classification, development, and comparison of fingerprints for identification |
What are the three layers of the skin? | Epidermis, dermis, hypodermis |
What is the function of the dermis? | It contains blood vessels and provides nutrients to the epidermis |
What are the three basic principles of fingerprints? | Individual characteristics, remain unchanged, have ridge patterns for classification |
What are the three basic ridge patterns of fingerprints? | Loops (60%), whorls (35%), arches (5%) |
What is the Henry System? | A fingerprint classification system used by Scotland Yard and modified by the FBI |
What are the eight fingerprint patterns in the FBI system? | Plain arch, tented arch, ulnar loop, radial loop, double loop, plain whorl, central pocket loop, accidental |
What are inked fingerprint impressions? | Prints made by rolling fingers to show the entire friction surface |
What are palm prints? | Prints made by the whole hand, often found at crime scenes |
What are major case prints? | Complete hand prints, including all parts of the hand |
What are latent prints? | Prints left by sweat, oil, or other substances on surfaces, often needing special techniques to be seen |
What are indented prints? | Prints that are pressed into a surface |
How are latent prints developed? | Using powders, chemicals, and lighting techniques |
What types of powders are used to develop fingerprints? | Regular, magnetic, and fluorescent powders |
What is Small Particle Reagent (SPR)? | A physical technique using small black particles to reveal fingerprint residues on various surfaces |
What is Ninhydrin used for? | Developing latent prints on porous surfaces by reacting with amino acids |
What is DFO? | A ninhydrin analogue that develops more prints than ninhydrin alone |
What is Sticky Side Powder? | A chemical method used to develop prints on adhesive surfaces |
What is Iodine Fuming? | A technique using iodine fumes to develop prints, especially on greasy surfaces |
What is Cyanoacrylate Fuming? | A method where super glue fumes react with latent prints, making them visible |
What are some mechanical methods for detecting latent prints? | Electrostatic detection, X-ray detection, and vacuum metal deposition |
What is the sequence for processing smooth surfaces? | Visual examination, UV light, non-magnetic powder, magnetic powder, fluorescent dye staining |
What is the sequence for processing porous surfaces? | Visual examination, fluorescent examination, iodine fuming, magnetic powder, DFO, ninhydrin, PD |
What is a firearm? | A firearm is any weapon that uses gunpowder or other explosive materials to launch a projectile |
What are the basic parts of a firearm? | Barrel, trigger, action, stock, and magazine |
What are the different types of firearms? | Handguns, rifles, and shotguns |
What is a handgun? | A firearm designed to be fired with one hand |
What is a rifle? | A firearm with a long barrel designed to be fired from the shoulder |
What is a shotgun? | A firearm designed to fire a spread of shot or a single projectile |
What is the difference between semi-automatic and fully automatic firearms? | Semi-automatic fires one round per trigger pull, while fully automatic fires continuously when the trigger is held |
What is gunpowder? | A mixture of chemicals, including potassium nitrate, sulfur, and charcoal, used as a propellant |
What is recoil? | The backward movement of a firearm when it is discharged |
What is the purpose of a safety on a firearm? | The safety prevents the firearm from firing accidentally |
What is muzzle velocity? | The speed at which a bullet leaves the barrel of a firearm |
What is a silencer or suppressor? | A device attached to the muzzle to reduce noise and muzzle flash when firing |
What is the difference between a bullet and a cartridge? | A bullet is the projectile, while a cartridge includes the bullet, casing, gunpowder, and primer |
What is the role of the primer in a cartridge? | The primer ignites the gunpowder, initiating the combustion that propels the bullet |
What is a rifled barrel? | A barrel with spiral grooves that spin the bullet, improving accuracy |
What are the basic safety rules for handling firearms? | Treat every firearm as loaded, never point at anything not meant to be destroyed, keep your finger off the trigger, and be sure of your target and what's beyond it |
How are firearms and toolmarks similar? | Both involve the comparison of marks on metal and may require casts of impressions to be made |
What materials are used to make casts of toolmarks? | Clay dam, epoxy, or plastic casting material |
What do toolmarks result from? | Contact between a tool and another surface |
What are class characteristics of toolmarks? | Shape and dimensions of the tool’s working surface, such as the distance between the teeth of a saw |
What are individual characteristics of toolmarks? | Features produced during manufacture or resulting from wear and tear |
What are the three types of toolmarks? | Compression (indented) toolmarks, sliding toolmarks, and cutting toolmarks |
How are compression toolmarks made? | A tool is pressed into a softer material, showing the outline of the working surface of the tool |
What are sliding toolmarks? | Marks created when a tool slides along a surface, typically with parallel striations |
What are cutting toolmarks? | A combination of compression and sliding toolmarks, where the tool indents and slides over the material being cut |
What surfaces produce better toolmark impressions? | Softer, smoother surfaces |
What surfaces are poor for toolmark retention? | Raw wood and hard metal |
How should toolmarks be processed at a crime scene? | Documented with notes, sketches, and photographs, and items should be collected or cast if they cannot be transported |
How do examiners compare toolmarks? | They make test toolmarks with the submitted tool and compare them microscopically to the crime scene marks, focusing on class and individual characteristics |
What can toolmarks on manufactured items reveal? | Information about the lot number, location of manufacture, or sale, such as hammer marks on nails or machine marks on metal shavings |
What is a positive identification in toolmark analysis? | The class and individual characteristics match, making it highly unlikely another tool made the mark |
What is a negative identification in toolmark analysis? | The toolmark does not match the submitted tool, and no microscopic comparison is necessary |
What does inconclusive mean in toolmark analysis? | Class characteristics match, but individual characteristics are insufficient to confirm a match |
What challenges do firearm and toolmark examinations face? | They are under scrutiny for subjectivity, though courts have ruled that subjectivity doesn't render them unscientific |
What are preliminary tests in toxicology? | They are rapid, simple tests used for drug or toxicology identification, often using color changes in reaction with reagents |
What are the advantages of color tests in toxicology? | They are rapid, require minimal sample, minimal training, and no sophisticated equipment |
What can a negative result in a color test indicate? | It may rule out a specific drug or intoxication |
What substances can be used for drug color tests? | Dry samples, aqueous solutions, solvent extracts, and chromatography |
What is Dragendorff’s Reagent used for? | It identifies alkaloidal bases and amines, producing an orange to brown color |
What does Duquenois Reagent identify? | It is used for marijuana, hashish, and THC, producing a gray to violet-blue color |
What can affect the stability of a drug sample? | Excipients, diluents, and cutting agents can change surface pH or react with the drug |
What is the key feature to identify marijuana microscopically? | The presence of glandular trichomes containing cannabis oil |
What are the three types of hairs found on cannabis plants? | Covering hairs, cystolithic hairs, and glandular hairs |
What is the microcrystal test used for? | It identifies chemical substances by observing crystal formation using a polarizing microscope |
What is the difference between direct and indirect microcrystal tests? | Direct tests produce crystals directly from the test substance, while indirect tests involve reagent reactions or failures to react |
What are common reagents used in microcrystal tests? | Acid, salt, or base solutions that precipitate alkaloids and other compounds |
What are the advantages of microcrystal tests? | They are sensitive, selective, rapid, and conserve evidence |
What are the disadvantages of microcrystal tests? | Impurities in samples can cause misleading results, and they require significant training and experience |
How are microcrystals examined? | Through polarized light microscopy to observe size, form, and color of crystals |
What factors can distort the crystal form in microcrystal testing? | Changes in temperature, humidity, reagent concentration, and test substance concentration |
What is the principle behind immunoassays? | Immunoassays work on the competition between a drug (analyte) and a labeled drug or antibody specific for the drug of interest |
What are the two main characteristics that immunoassays need to exhibit? | Specificity and sensitivity |
What are the main components of immunoassays? | Antibodies, antigens, molecular labels, and substrate molecules |
What is the principle of a competitive binding assay? | In a competitive binding assay, the proportion of labeled drug bound to the antibody is inversely proportional to the concentration of the unlabeled drug in the sample |
What are UV-Visible Spectrophotometry used for? | UV-Visible Spectrophotometry is used for quantifying and characterizing drugs, diluents, metabolites, and other substances by measuring their absorption of electromagnetic radiation |
How does Infrared Spectrophotometry work? | Infrared Spectrophotometry measures the absorption of infrared radiation by molecules, causing transitions between rotational and vibrational energy levels |
What is chromatography? | Chromatography is a technique used to separate chemical compounds or analytes |
What is the basic principle of Gas Chromatography (GC)? | In GC, analytes are separated based on their partitioning between an inert gas (mobile phase) and a liquid stationary phase, with different compounds having different retention times |
What is High-Performance Liquid Chromatography (HPLC)? | HPLC separates chemical components by injecting a small sample onto a column, where the components are detected and quantified |
How does Mass Spectrometry (MS) work? | Mass Spectrometry analyzes ions formed by bombarding a sample with electrons and measures their mass-to-charge ratio, providing a characteristic spectrum for compound identification |
How are drugs identified in forensic toxicology? | Drugs are identified by verifying packaging, chain of custody, weighing the sample, and performing confirmatory tests like IR or GC-MS |
What is the role of the confirmatory test in drug analysis? | Confirmatory tests, like IR and GC-MS, are used to definitively identify a drug by proving its structure |
What are some common drugs found in forensic toxicology? | Cocaine, heroin, and marijuana |
How is cocaine usually found? | Cocaine is found as cocaine HCl (powder) or crack cocaine (free base) |
How is heroin commonly used? | Heroin is used as a white/brown powder or black tar, and is often injected or smoked |
What is marijuana typically tested with? | Marijuana is tested using microscopy, color tests, and thin layer chromatography |
How are bodily fluids typically examined in forensic toxicology? | Bodily fluids are examined by noting the weight/volume, checking for tampering, and screening with immunoassays before confirmatory testing like GC-MS |
What are biological stains? | Originate from biological fluids and provide an indication of the nature of a stain through rapid chemical color tests |
What is a presumptive test? | It provides an indication of a stain's nature but cannot positively identify it, requiring further confirmatory tests |
What are the components of blood? | Red blood cells (RBCs), white blood cells (leukocytes), platelets, plasma, and serum proteins |
How does blood clot? | Blood clots when the dissolved protein fibrinogen is converted into fibrin |
What is the role of red blood cells? | They oxygenate tissues by transporting oxygen through the oxygen-hemoglobin complex |
What are white blood cells responsible for? | White blood cells are involved in the body’s response to infection, with lymphocytes responsible for antibody production |
What is plasma? | The cell-free fraction of blood containing proteins, salts, glucose, hormones, and vitamins |
What does the physical nature of bloodstains indicate? | It can provide useful information about the nature and movement of blood at a crime scene |
What is a common color test for blood? | The Kastle-Meyer test, which turns pink when it reacts with blood |
What does luminol cause when it reacts with blood? | It causes stains to luminesce (fluoresce) |
What is the Kastle-Meyer test used for? | To detect blood using phenolphthalein, hydrogen peroxide, and alcohol |
How does the Takayama or hemochromogen test confirm blood? | It reacts with ferrous iron hemoglobin to produce red feathery crystals observable under a microscope |
What are some confirmatory tests for blood? | Microscopic analysis and spectroscopy, which can identify hemoglobin derivatives like methemoglobin |
What does the Hematrace test detect? | The presence of human hemoglobin in a sample, similar to a pregnancy test |
What are the components of semen? | Spermatozoa and seminal plasma, which contains proteins, salts, and organic substances |
What does seminal plasma contain? | Flavins (which cause UV fluorescence), choline, and proteins from the seminal vesicles and prostate |
How can semen be visually detected? | Semen forms a white or yellow stain and may require alternative light sources to be visible |
What does the acid phosphatase (AP) test indicate? | It indicates the presence of semen, as it reacts with sodium alpha-naphthyl phosphate and Fast Blue B to form a purple color |
What does the PSA test detect? | Prostate-specific antigen (PSA), a protein found in high concentration in human semen |
What is the purpose of the Christmas tree stain in semen analysis? | To stain sperm, with Nuclear Fast Red for the head and picric acid for the tail |
What is the TSI interval used for? | To estimate how long sperm has been present in the vagina, aiding in distinguishing between assault and consensual intercourse |
How does saliva appear when dried? | It leaves a faint trace that can be detected under alternative light sources |
How is saliva detected? | Using a reagent like amylose-procion red on blotting paper, which changes color in contact with saliva |
What is the main component of urine? | Urea and creatinine, with a distinctive ammonia-like odor |
How is urine detected? | Using the Urea litmus paper test, which reacts with ammonia to change color |
What does the Jaffe test detect in urine? | Creatinine, a breakdown product of creatine, which forms a red compound with picric acid |
What does feces contain? | Unabsorbed residues, food residues, gut wall debris, digestive secretions, and bile pigment breakdown products |
What does the Edelman test detect in feces? | Urobilinogen, a bile pigment, which reacts with mercuric chloride to form a pink-red product |
How is feces analyzed microscopically? | By identifying undigested food residues and potential parasitic organisms |