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Lab Microbiology
Question | Answer |
---|---|
Group of bacteria forms a | Colony |
Disease causing microorganisms | Pathogens |
4 types of pathogens | Fungi, Parasites, Viruses, Bacteria |
Color of bacteria that stain gram positive | Purple |
Mordant | Iodine |
Retains safranin red | Gram Negative |
Microorganisms normally found in the body and is not pathogenic | Normal Flora |
In the absence of oxygen | Anaerobic |
Study of fungi | Mycology |
Requires living host to multiply and contains either RNA or DNA | Virus |
Sexually transmitted disease sometimes found in urine | Trich |
Test used to recover pinworms | Cellophane/Tape Test |
Used to kill or inhibit the growth of a bacterial infection | Antibiotic |
Total absence of microorganisms | Sterile |
Contains ingredients that inhibit the growth of certain microorganisms | Selective Media |
The media on which the specimen is first inoculated | Primary Media |
Counterstain | Safranin |
Requires very specific nutrient and environmental conditions | Fastidious |
Decolorizer | Alcohol/Acetone |
Stain used to differentiate bacteria by their cell walls | Gram Stain |
Coccidioides, immitis, and Aspergillus are types of | Fungi |
Beta hemolysis is characterized by | A clear zone around the colony. Complete destruction of erythrocytes |
Beta hemolysis indicates | Group A Strep |
Alpha hemolysis is characterized by | Green discoloration around the colony. Due to incomplete hemoglobin breakdown |
Alpha hemolysis indicates | Group B Strep/Normal Flora |
Test used to differentiate Staph from Strep | Oxidase Test (Hydrogen Peroxide) |
Intestinal protozoan parasite that causes malabsorption syndrome | Giardia Lamblia |
Parasitic worms are called | Helminth |
Pathogenic bacteria found in a wound | Staphaureus |
Purple gram stain reaction | Gram Positive |
Patients should avoid aspirin and vitamin C for how long | 7 and 3 days |
Test performed to differentiate staph organisms | Coagulase |
Most common bacteria recovered from urine | E. coli |
Specimen that is inoculated right from the source of infection | Direct Culture |
Procedure to measure the growth of bacteria in the presence of antibiotics | Antibiotic Sensitivity/Susceptibility Testing |
Scientific name for the pinworm | Enterobius Vermicularis |
Test done for the most effective antibiotic | Kirby Bauer |
Comma or Spiral shaped bacteria | Spirilla |
Round or oval bacteria | Cocci |
Rod-shaped bacteria | Bacilli |
Cocci that appear singly | Micrococci |
Cocci that appear in pairs | Diplococci |
Cocci that appear in grapelike clusters | Staphylococci |
Cocci that appear in chains | Streptococci |
Pneumonia is caused by what type of bacteria (shape) | Cocci |
Strep Throat is caused by what type of bacteria (shape) | Cocci |
Wound Infection is caused by what type of bacteria (shape) | Cocci |
Food Poisoning is caused by what type of bacteria (shape) | Cocci |
Toxic Shock Syndrome is caused by what type of bacteria (shape) | Cocci |
Gonorrhea is caused by what type of bacteria (shape) | Cocci |
Meningitis is caused by what type of bacteria (shape) | Cocci |
Bacillus found side by side like boards | Palisades |
Bacillus attached end to end in chains | Streptobacilli |
Botulism is caused by what type of bacteria (shape) | Bacilli |
TB is caused by what type of bacteria (shape) | Bacilli |
Typhoid fever is caused by what type of bacteria (shape) | Bacilli |
Diptheria is caused by what type of bacteria (shape) | Bacilli |
UTI is caused by what type of bacteria (shape) | Bacilli |
Plague is caused by what type of bacteria (shape) | Bacilli |
Legionnaires’ is caused by what type of bacteria (shape) | Bacilli |
Tetanus is caused by what type of bacteria (shape) | Bacilli |
Gangrene is caused by what type of bacteria (shape) | Bacilli |
Syphilis is caused by what type of bacteria (shape) | Spirillum/Spirochete |
Lyme Disease is caused by what type of bacteria (shape) | Spirillum/Spirochete |
Pyloric Ulcers (H. Pylori) is caused by what type of bacteria (shape) | Spirillum/Spirochete |
Food poisoning in US is caused by what type of bacteria (shape) | Spirillum/Spirochete |
Streptococcus is gram | positive |
Staphylococcus is gram | positive |
Klebsiella is gram | negative |
E. coli is gram | negative |
Gonorrhea is gram | negative |
Bacteria grow at what temp? | 37 Degrees Celsius |
Incubate bacteria for how many hours? | 24-72 hours |
Aerobic Bacteria | Require Oxygen |
Anaerobic Bacteria | Don’t like Oxygen |
Aspergillus is a type of | fungi |
Candida is also known as | yeast |
Coccidioides Immitis is also known as | valley fever |
Skin disease caused by fungi | Tinea (Ring Worm), Athlete’s Foot, Jock Itch |
Fungi causes what type of pneumonia | pneumocysitis |
4 Mycology Testing Methods | Skin scraping/swabs, KOH Prep, Wet Prep, Sabourad’s dextrose agar |
Testing methods for virology | cell culture, ELISA, Kit tests (flue, herp, rubella) |
Giardia lamblia is caused by | contaminated water |
Plasmodium causes | malaria |
Pubic lice is called | pthirus pubis |
Head lice is called | pediculus humanus |
Common intestinal parasites | Tape worm, round worm, hook worm, pin worm |
Testing method for intestinal parasites | Formalin preserved stool specimen for microscopic exam |
Testing method for malaria | thin & thick blood smear |
Testing method for trich | Urine & Vaginal Exam |
Contains substances that visibly change as a result of the metabolic activity of certain organisms | Indicator (Differential) Media |
Types of selective and indicator media | EMB, MacConkey’s |
CNA is a type of _________ media | selective |
A culture that is grown from a single colony taken from a direct culture | Pure Culture |
Swab of affected area is placed on a microscope slide and gram stained | Direct Smear |