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Lab Microbiology

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Question
Answer
Group of bacteria forms a   Colony  
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Disease causing microorganisms   Pathogens  
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4 types of pathogens   Fungi, Parasites, Viruses, Bacteria  
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Color of bacteria that stain gram positive   Purple  
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Mordant   Iodine  
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Retains safranin red   Gram Negative  
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Microorganisms normally found in the body and is not pathogenic   Normal Flora  
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In the absence of oxygen   Anaerobic  
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Study of fungi   Mycology  
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Requires living host to multiply and contains either RNA or DNA   Virus  
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Sexually transmitted disease sometimes found in urine   Trich  
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Test used to recover pinworms   Cellophane/Tape Test  
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Used to kill or inhibit the growth of a bacterial infection   Antibiotic  
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Total absence of microorganisms   Sterile  
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Contains ingredients that inhibit the growth of certain microorganisms   Selective Media  
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The media on which the specimen is first inoculated   Primary Media  
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Counterstain   Safranin  
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Requires very specific nutrient and environmental conditions   Fastidious  
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Decolorizer   Alcohol/Acetone  
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Stain used to differentiate bacteria by their cell walls   Gram Stain  
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Coccidioides, immitis, and Aspergillus are types of   Fungi  
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Beta hemolysis is characterized by   A clear zone around the colony. Complete destruction of erythrocytes  
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Beta hemolysis indicates   Group A Strep  
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Alpha hemolysis is characterized by   Green discoloration around the colony. Due to incomplete hemoglobin breakdown  
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Alpha hemolysis indicates   Group B Strep/Normal Flora  
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Test used to differentiate Staph from Strep   Oxidase Test (Hydrogen Peroxide)  
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Intestinal protozoan parasite that causes malabsorption syndrome   Giardia Lamblia  
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Parasitic worms are called   Helminth  
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Pathogenic bacteria found in a wound   Staphaureus  
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Purple gram stain reaction   Gram Positive  
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Patients should avoid aspirin and vitamin C for how long   7 and 3 days  
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Test performed to differentiate staph organisms   Coagulase  
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Most common bacteria recovered from urine   E. coli  
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Specimen that is inoculated right from the source of infection   Direct Culture  
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Procedure to measure the growth of bacteria in the presence of antibiotics   Antibiotic Sensitivity/Susceptibility Testing  
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Scientific name for the pinworm   Enterobius Vermicularis  
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Test done for the most effective antibiotic   Kirby Bauer  
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Comma or Spiral shaped bacteria   Spirilla  
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Round or oval bacteria   Cocci  
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Rod-shaped bacteria   Bacilli  
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Cocci that appear singly   Micrococci  
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Cocci that appear in pairs   Diplococci  
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Cocci that appear in grapelike clusters   Staphylococci  
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Cocci that appear in chains   Streptococci  
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Pneumonia is caused by what type of bacteria (shape)   Cocci  
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Strep Throat is caused by what type of bacteria (shape)   Cocci  
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Wound Infection is caused by what type of bacteria (shape)   Cocci  
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Food Poisoning is caused by what type of bacteria (shape)   Cocci  
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Toxic Shock Syndrome is caused by what type of bacteria (shape)   Cocci  
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Gonorrhea is caused by what type of bacteria (shape)   Cocci  
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Meningitis is caused by what type of bacteria (shape)   Cocci  
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Bacillus found side by side like boards   Palisades  
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Bacillus attached end to end in chains   Streptobacilli  
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Botulism is caused by what type of bacteria (shape)   Bacilli  
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TB is caused by what type of bacteria (shape)   Bacilli  
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Typhoid fever is caused by what type of bacteria (shape)   Bacilli  
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Diptheria is caused by what type of bacteria (shape)   Bacilli  
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UTI is caused by what type of bacteria (shape)   Bacilli  
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Plague is caused by what type of bacteria (shape)   Bacilli  
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Legionnaires’ is caused by what type of bacteria (shape)   Bacilli  
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Tetanus is caused by what type of bacteria (shape)   Bacilli  
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Gangrene is caused by what type of bacteria (shape)   Bacilli  
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Syphilis is caused by what type of bacteria (shape)   Spirillum/Spirochete  
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Lyme Disease is caused by what type of bacteria (shape)   Spirillum/Spirochete  
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Pyloric Ulcers (H. Pylori) is caused by what type of bacteria (shape)   Spirillum/Spirochete  
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Food poisoning in US is caused by what type of bacteria (shape)   Spirillum/Spirochete  
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Streptococcus is gram   positive  
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Staphylococcus is gram   positive  
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Klebsiella is gram   negative  
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E. coli is gram   negative  
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Gonorrhea is gram   negative  
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Bacteria grow at what temp?   37 Degrees Celsius  
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Incubate bacteria for how many hours?   24-72 hours  
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Aerobic Bacteria   Require Oxygen  
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Anaerobic Bacteria   Don’t like Oxygen  
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Aspergillus is a type of   fungi  
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Candida is also known as   yeast  
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Coccidioides Immitis is also known as   valley fever  
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Skin disease caused by fungi   Tinea (Ring Worm), Athlete’s Foot, Jock Itch  
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Fungi causes what type of pneumonia   pneumocysitis  
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4 Mycology Testing Methods   Skin scraping/swabs, KOH Prep, Wet Prep, Sabourad’s dextrose agar  
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Testing methods for virology   cell culture, ELISA, Kit tests (flue, herp, rubella)  
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Giardia lamblia is caused by   contaminated water  
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Plasmodium causes   malaria  
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Pubic lice is called   pthirus pubis  
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Head lice is called   pediculus humanus  
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Common intestinal parasites   Tape worm, round worm, hook worm, pin worm  
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Testing method for intestinal parasites   Formalin preserved stool specimen for microscopic exam  
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Testing method for malaria   thin & thick blood smear  
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Testing method for trich   Urine & Vaginal Exam  
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Contains substances that visibly change as a result of the metabolic activity of certain organisms   Indicator (Differential) Media  
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Types of selective and indicator media   EMB, MacConkey’s  
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CNA is a type of _________ media   selective  
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A culture that is grown from a single colony taken from a direct culture   Pure Culture  
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Swab of affected area is placed on a microscope slide and gram stained   Direct Smear  
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