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Clinical Laboratory
Lab Questions from N.S.
| Question | Answer |
|---|---|
| What are the Standard Form (SF) series numbers laboratory request forms? | The 500 series. |
| Where are laboratory reports of patients placed? | Reports of inpatients are placed in the inpatient health record, while laboratory reports on outpatients are placed in the outpatient health record. |
| With what exception, SF laboratory forms are multicopied and precarbonized for convenience? | The SF-545, Laboratory Report Display |
| Where are the carbon copies of the laboratory report forms filed? | In the laboratory's master file. |
| What directive contains a complete listing of all standard forms (SF) and their purposes? | Manual of the Medical Department, (MANMED), NAVMED P-117 |
| What form is used to request a urinalysis test? | SF-550, Urinalysis Request Form. |
| What part of the SF-550 Urinalysis Request Form would you enter "Clean catch midstream to R/O urinary tract infection," if applicable? | In the "Remarks" block. |
| How should a standard form that is used to record test results, be attahed to the SF-545, Laboratory Record Display, inside the patient's health record? | In chronological order. |
| What are two principal methods of obtaining blood samples? | Finger Puncture and Venipuncture. |
| What part of the microscope is the the platform on which a preparation is placed for examination? | The stage (of the framework). |
| What should NEVER be used to clean microscope lenses? | Alcohol or similar solvent. |
| What are the 5 test involved in a complete blood count? | Total RBC count; hemoglobin determination; hematocrit reading; total white blood cell (WBC) count; and the differential WBC. |
| Which RBCs should NOT be counted in the manual Sahli Pipette blood count method? | Those touching the lines on the right and at the bottom. |
| What is needed to manually count red blood cells (erythrocytes) and white blood cells (leukocytes)? | A microscope and an instrument called a hemacytometer. |
| What is the hemacytometer? | A thick glass slide with three raised parallel platforms on the middle third of the device. |
| What is the composition of the central platform of the hemacytometer? | It is subdivided by a transverse groove to form two halves, each wider than the two lateral platforms and separated from them and from each other by moats. |
| What hemacytometer grid is used by most laboratories for counting blood cells? | The Improved Neubauer Ruling. |
| How are counts of RBCs and WBCs each expressed? | As concentration: cells per unit volume of blood. |
| Why is the unit of volume for cell counts expressed as cubic millimeters? | Because of the linear dimensions of the hemacytometer chamber. |
| What is the total red blood cell (erythrocyte) count? | The number of red cells in one cubic millimeter of blood |
| What method is used to manually count red blood cells? | The Unopette Method. |
| What is used in the Unopette procedure for manually counting red blood cells? | A disposable diluting pipette system that provides a convenient, precise, and accurate method for obtaining a red blood cell count. |
| What materials are needed to perform the Unopette Method of counting red blood cells? | A disposable Unopette, hemacytometer and coverglass, microscope with light source, hand-held counter, and a laboratory chit. |
| What is the first step in the Unopette procedure for counting red blood cells? | Puncture the diaphragm in the neck of the diluent reservoir with the tip of the capillary shield on the capillary pipette. |
| When is filling of the Unopette pipette complete and will stop automatically? | When the blood reaches the end of the capillary bore in the neck of the pipette. |
| In the Unopette Method for counting red blood cells, how much blood is discarded from the pipette, before loading the counting chamber of the hemacytometer? | The first three drops. |
| What action is taken when counting cells in each field that touch the lines on the right or at the left bottom of the hemacytometer? | Don't count them. |
| What is indicated in counting red blood cells if each field of the hemacytometer varies by more than 20? | Probably indicates a poor distribution of the cells in the fluid, resulting in an inaccurate count, and the test must be repeated. |
| What is the formula for determining the number of RBCs per cubic millimeter of blood when using the hemacytometer? | Total the number of cells counted in all five fields and multiply by 10,000. |
| What is hemoglobin's main function in the body? | To carry oxygen from the lungs to the tissues and to assist in transporting carbon dioxide from the tissues to the lungs. |
| What is hemoglobinometry? | The measurement of the concentration of hemoglobin in the blood. |
| Where does the formation of hemoglobin take place? | In the developing red cells located in bone marrow. |
| How does dehydration affect hemoglobin? | It results in above-normal hemoglobin values. |
| What is the most widely used automated method for testing for hemoglobin values? | The cyanmethemoglobin method. |
| What are the manual methods for determining blood hemoglobin? | The Haden-Hausse and Sahli-Hellige methods. |
| What determines the percentage of RBCs in whole blood? | The hematocrit or packed cell volume (PCV). |
| What is the most accurate manual method of determining the hematocrit or packed cell volume? | The microhematocrit method. |
| What is the first step in performing the microhematocrit method of determining packed cell volume? | Fill the capillary tube two-thirds to three-quarters full with well-mixed, oxalated venous blood or fingertip blood. |
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