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Micro, lab 22
Micro 204, Hydrogen sulfide production lab 22
Question | Answer |
---|---|
When hydrogen sulfide is released form an organism it is in the form of a X. | gas |
The production of hydrogen sulfide is an X reaction. | anaerobic |
UNder natural conditions where you might expect find hydrogen sulfide produced by organisms? | Mud in swamps, lakes, streams |
X is the color of a positive hydrogen sulfide test. | Black |
In order to produce hydrogen sulfide, microorganisms must posses the enzymes to attack X containing compounds. | sulfur |
The formation of the compound X provides the basis for the detection of hydrogen sulfide. | iron sulfide |
List four media used to detect hydrogen sulfide production. | peptone iron agar (PIA) kligler iron agar (KIA) triple sugar iron agar (TSI) lead acetate agar |
X is the salt added to the medium to detect hydrogen sulfide production. | Ferrous salt |
X is the pH indicator used in KIA medium. | Phenol red |
The acid colors of carbohydrates fermentation reactions must be read at X hours at X degrees C to be valid. | 24, 37 |
X and X are considered to be multipurpose types of media. | Kligler iron and triple sugar, (KIA) and (TSA) |
In the absence of sugars, does protein decomposition result on a shift toward the acid or alkaline side? | The production of acid requires sugar |
Medium used: | Kligler iron agar (KIA) |
Test positive means: | black precipitate, means hydrogen sulfide was produced |