FYP
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show | The diacylglycerol O-acyltransferase (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis.
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show | An association between lysine at amino acid position 232 with elevated milk fat content, while an alanine at this position is associated with lowered milk fat content
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show | Any difference in the nucleotide sequence- Variations
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show | A technique that allows exponential amplification of DNA sequences. Genes are targeted with complimentary primers to the specific sequence of DNA and amplified with an enzyme known as polymerase
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show | A technique that finds variations in DNA by using an enzyme to break down the DNA different sizes depending on its specific code, the process is then carried to Gel Electrophoresis
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show | Separates DNA fragment made by RFLP by size and charge. Longer pieces of DNA stay close to wells, shorter DNA ,moves closer to positive charge creating unique fingerprint.
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show | DNA is cut and inserted into plasmid DNA, this is then in bacterial cells. DNA is isolated and added to a sequencing plate. DNA basses are added, polymerase is added, DNA primers, and fluorescent terminator bases are added.PCR then Electrophoesis occurs
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Real-time PCR (Polymerase Chain Reaction) | show 🗑
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SYBR Green Assays | show 🗑
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show | G
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show | I
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show | G
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show | N
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show | X
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show | Y
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Extension | show 🗑
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411bp fragment | show 🗑
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Restriction sites for a restriction Enzyme | show 🗑
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show | S
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show | C
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10433-10444 position of the K232A Polymorphism | show 🗑
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show | Q
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Threshold Cycle | show 🗑
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show | H
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show | H
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Clean and Concentrator -25TM Kit | show 🗑
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show | The building blocks of nucleic acids; they are composed of three subunit molecules: a nitrogenous base, a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group
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