Food Micro Lab Final Exam
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| Define virus. | An obligate intracellular parasite.
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| T or F: Viruses can only replicate with a host cell. | True
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| T or F: Viruses have their own metabolic capacity. | False
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| The genome of a virus is surrounded by a ____ protein layer. | capsid
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| Name thee human enteric viruses. | Norovirus, Hepatitis A, Astrovirus, Rotavirus
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| T or F: Viruses prefer alkaline pH. | True
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| Name two ways which viruses are concentrated. | Ultrafiltration, 10%PEG6000 + 0.3 M NaCl precipitation
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| How are viruses analyzed? | Plaque assay or harvest RNA for qRT-PCR
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| What are three methods for human norovirus detection? | Electron microscopy, RT-PCR, enzyme immunoassay
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| T or F: Electron microscopy can distinguish between strains of viruses. | False
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| ____ is the method of choice for detecting low virus titers. | RT-PCR
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| T or F: RT-PCR can distinguish between virus genogroups. | True
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| What are four desirable characteristics for norovirus detection methods? | Sensitive and specific, rapid results, can be used for detection and genotyping, works on a variety of sample types, can distinguish between infectious and non-infectious
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| The ISO/CEN method can quantify what viruses? | Hepatitis A and norovirus
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| T or F: There is no standardized methods for the diagnosis of enteric viruses. | True
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| Name two traditional ligands for human norovirus recognition. | Monoclonal antibodies, polyclonal sera
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| Name three novel ligands for human norovirus recognition. | Single chain monoclonal antibodies, peptide antigens, nucleic acid aptamers, HBGA
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| Single chain antibodies are often concentrated and purified to use in ____. | EIA
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| Quantification of viruses is done using ____. | RT-PCR
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| Low levels of viruses can lead to variation of up to __ log. | 1
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| T or F: Viruses in foods are evening distributed. | False
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| T or F: There is no regulatory microbiological data available for viruses. | True
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| Infectivity assays reduced ____ and increases _____. | inhibitors, sensitivity
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| T or F: Infectivity assays can be used to grow norovirus. | False
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| Laboratory growth of viruses requires ____ cells. | host
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