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HSF II Lab II
Lab Exercise 2
| Question | Answer |
|---|---|
| Hemacytometer | Counting blood cells on a grid under a microscope with diluted blood. Normal RBC found 4.5-5.0 X 10^6 per mm3 |
| Polycythemia | increase in number of RBCs |
| Anemia | decreased oxygen-carrying capacity of blood |
| Hematocrit or Packed Cell Volume | used to determine if anemia present. Centrifuging whole blood separating formed elements and platelets and plasma. Normal ranges males 41-52%, females 37-47% |
| Hemoglobin | RBC protein for oxygen transport |
| Hemoglobin Concentration | depends on hydration status of the patient. Used Tallquist Method. Normal range males 14-18 g/dl (grams per 100mL) females 12-16g/dl |
| Tallquist Method | for hemoglobin concentration. place a drop of blood on special absorbent paper, once dried, compare with colors from the Tallquist card. |
| Mean Corpuscular Volume (MCV) | average volume of an individual RBC. Normal range 80-100 fL (femtoliters = 10^-15) |
| Normocytic, Macrocytic, Microcytic | normal sized cells, big cells, little cells |
| Mean Corpuscular Hemoglobin (MCH) | average mass of hemoglobin in a typical RBC. Normal values 27-31 picograms per cell |
| Calculate MCV | MCV = (Hematocrit)/[RBC} [RBC] = 5X10^6 RBC/microliter |
| Calculate MCH | MCH = [hemoglobin(g/dl) X 10dl/L]/[RBCcount per liter] |
| Iron Deficiency Anemia | RBCs are microcytic with low hemoglobin per cell |
| Vitamin B12 Deficiency | RBCs are macrocytic with normal hemoglobin |
| ABO Blood Type | A= A antigens on RBC, Anti-B antigens in plasma B = B antigens on RBC, Anti-A antigens in plasma AB = AB antigens on RBC, no anti- antigens in plasma O no antigens on RBC or in plasma |
| Sickle-cell Anemia | genetic disease that results in the production of modified hemoglobin molecules, hemoglobin S. |
| Of the four types of tissue (muscle, epithelial, connective, and nervous), blood falls into which category? | connective |
| An increase in the total # of RBCs is [blank], while a decrease is referred to as [blank]. | polycythemia and anemia |
| What is the correlation between the total number of RBCs and the oxygen -carrying capacity of blood? | As total number of RBCs increases, there is an increased oxygen-carrying capacity |
| After you have counted a representative sample of RBCs, what next step should you take to obtain the RBCs per mm3? | multiply by the dilution factor |
| An abnormally high hematocrit level may be caused by either polycythemia or [blank]. | dehydration |
| What role does hemoglobin play in metabolism? | oxygen transport |
| Name the method used to determine hemoglobin concentration in whole blood by placing a drop of blood on a piece of paper and comparing it to standard color swatches | Tallquist Method |
| An RBC with a larger than normal volume is referred to a macrocytic, while an RBC with a smaller than normal volume is microcytic: true or false | True |
| Calculate mean corpuscular volume | divide hematocrit (as a decimal) by the concentration of RBCs per liter |
| How does calculating the hemoglobin concentration differ from calculating the mean corpuscular hemoglobin? | MCH refers to average mass of hemoglobin in a single RBC, where hemoglobin concentration refers to mass of hemoglobin in whole blood |
| A patient presents to the ED with lightheadedness after a recent injury involving blood loss. The pt has a known diagnosis of chronic anemia. What is one lab test you could order to assess her current condition? | Total red blood cell count, hematocrit, or hemoglobin concentration |
| What abnormalities would you expect to see in MVC/MCH in a patient with iron deficiency anemia? | microcytic RBCs and low hemoglobin concentrations per cell |
| What abnormalities would you expect to see in MVC/MCH in a patient with Vitamin B12 deficiency? | Macrocytic RBCs with normal hemoglobin concentrations per cell |
| An individual with Type B blood would have [blank] antigens on the surface of the RBCs and make anti-[blank] antibodies | B and A |
| An individual with Type AB blood would have [blank] antigens on the surface of the RBCs and make anti-[blank] antibodies | A+B and none |
| All those individuals with Rh- blood carry anti-Rh antibodies in the blood: true or false | false |
| What type of hemoglobin is found in the RBCs of sickle cell anemia patients, which cause their cells to take on a crescent shape when deoxygenated? | Hemoglobin S |
| What is the name for the counting chamber used in class during the total RBC count activity? | hemocytometer |
| After centrifuging a sample of whole blood, what material appears at the top of the capillary tube? | plasma |
| An abnormally low hematocrit level always means the patient is anemic: true or false | false, could be overhydration |
| Which blood types in a woman would be concerning if she was carrying her second pregnancy? | a negative blood type |
| The term [blank] refers to an overall increase in the total number of white blood cells, while [blank] refers to an overall decrease | leukocytosis and leukopenia |
| Which leukocyte contains a bilobed nucleus and red-orange cytoplasmic granules? | Eosinphils |
Created by:
connorquinby
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